| 苏丹草愈伤组织酚类物质提取工艺的研究 |
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| 引用本文： | 苏衍菁,刘苏娇,徐俊,赵国琦.苏丹草愈伤组织酚类物质提取工艺的研究[J].安徽农业科学,2012(30):14633-14635. |
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| 作者姓名： | 苏衍菁 刘苏娇 徐俊 赵国琦 |
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| 作者单位： | 扬州大学动物科技学院,江苏扬州,225009;扬州大学动物科技学院,江苏扬州,225009;扬州大学动物科技学院,江苏扬州,225009;扬州大学动物科技学院,江苏扬州,225009 |
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| 基金项目： | 扬州大学研究生创新基金项目 |
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| 摘 要： | 目的]探索苏丹草愈伤组织酚类物质的最佳提取工艺。方法]以3种不同类型的苏丹草愈伤组织为材料,采用Folin-Ciocalteu比色法研究了超声波破碎时间、乙醇浓度以及抽提次数对酚类物质提取效率的影响。结果]提取苏丹草愈伤组织酚类物质的最佳工艺条件为:抽提液乙醇浓度50%,超声波破碎时间32 min,抽提次数3次。3种不同类型的苏丹草愈伤组织中,Ⅱ型愈伤组织的酚类物质含量最高。结论]该研究为苏丹草的综合开发和深度利用提供技术支持。
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| 关 键 词： | 苏丹草 愈伤组织 Folin-Ciocalteu比色法 酚类物质 |
Study on Extraction Method of Polyphenols in Callus of Sorghum sudanense |
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| Institution: | SU Yan-jing et al(College of Animal Science and Technology,Yangzhou University,Yangzhou,Jiangsu 225009) |
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| Abstract: | Objective] To explore the optimum extraction for polyphenols in callus of Sorghum sudanense.Method] With three different kinds of S.sudanense callus as materials,effects of microwave time,ethanol concentration and tines on extraction efficiency were studied by using Folin-Ciocalteu colorimetry method.Result] The optimum conditions of phenols extraction for S.sudanense callus was ensured that the ultrasonic crushing time is 32 min,the ratio of solid to liquid is 1∶5,the ethanol concentration is 50% and the extraction time is 3.The II type callus has the highest polyphenol content.Conclusion] The study will provide technique support for comprehensive development and deeply utilization of S.sudanense. |
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| Keywords: | Sorghum sudanense Callus Folin-Ciocalteu colorimetry Phenols |
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