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进境韩国兰花细菌性褐腐病菌的分离与鉴定
引用本文:厉艳,王英超,尼秀媚,甘琴华,封立平.进境韩国兰花细菌性褐腐病菌的分离与鉴定[J].安徽农业科学,2012(31):15243-15244.
作者姓名:厉艳  王英超  尼秀媚  甘琴华  封立平
作者单位:山东出入境检验检疫局,山东青岛,266001;山东出入境检验检疫局,山东青岛,266001;山东出入境检验检疫局,山东青岛,266001;山东出入境检验检疫局,山东青岛,266001;山东出入境检验检疫局,山东青岛,266001
基金项目:国家质检总局科技计划项目(2010IK270)
摘    要:目的]鉴定进境韩国兰花细菌性褐腐病菌。方法]从韩国进境的大花蕙兰植株病变叶片中分离到细菌菌株,并通过形态鉴定、培养特征、生理生化及16S rDNA序列分析和致病性测定对其进行了鉴定。结果]确认该病菌为兰花细菌性褐腐病菌(Erwinia cypripe-dii),这是我国首次从进境兰花中检出该病害。结论]为我国兰花细菌性褐腐病菌的预防及控制奠定了基础。

关 键 词:大花蕙兰  兰花细菌性褐腐病菌  病原鉴定

Isolation and Identification of Erwinia cypripedii from Imported Korea Cymbidium
Institution:LI Yan et al(Shandong Entry-exit Inspection and Quarantine Bureau,Qingdao,Shandong 266001)
Abstract:Objective] The aim was to identify Erwinia cypripedii from imported Korea Cymbidium.Method] The pathogenic bacteria was isolated from imported Korea Cymbidium leaves,and then it was identified by morphological identification,cultural characteristics,physiological and biochemical reactions,16S rDNA gene sequences analysis and pathogenicity test.Result] The isolated strain was identified as E.cypripedii,and it was the first interception in China.Conclusion] The research result lays the foundation for the prevention and control of E.cypripedii in China.
Keywords:Cymbidium  Erwinia cypripedii  Pathogen identification
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