地衣芽孢杆菌BL03脂肪酶基因LIP的克隆及序列分析 |
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引用本文: | 刘生峰,肖进文,周蓓莉,周庆,聂福平,王昱,杨俊,李应国. 地衣芽孢杆菌BL03脂肪酶基因LIP的克隆及序列分析[J]. 安徽农业科学, 2012, 0(30): 14661-14662,14681 |
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作者姓名: | 刘生峰 肖进文 周蓓莉 周庆 聂福平 王昱 杨俊 李应国 |
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作者单位: | 重庆出入境检验检疫局,重庆400020;重庆市进出口食品安全工程研究中心,重庆400020;重庆出入境检验检疫局,重庆,400020;重庆出入境检验检疫局,重庆400020;西南大学,重庆400715 |
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基金项目: | 国家质检总局公益性项目(201010045);重庆市科技攻关项目(CSTC)(2011AC1056;2011AC1057) |
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摘 要: | [目的]克隆地衣芽孢杆菌BL03的脂肪酶基因LIP并对其序列进行分析。[方法]以地衣芽孢杆菌BL03基因组核酸为模板,PCR扩增得到地衣芽孢杆菌脂肪酶基因,用pMD19-T载体克隆后进行PCR鉴定及序列测定和生物信息学分析。[结果]测序结果表明该片段完整开放阅读框大小为615 bp,与已发表的地衣芽孢杆菌LTP基因(GeneID:3098655)相比,核酸序列相似为99.67%;推导编码氨基酸序列相似性为99.02%。[结论]该基因的获得为其异源表达及相关研究奠定了基础。
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关 键 词: | 地衣芽孢杆菌 脂肪酶基因 克隆 序列分析 |
Cloning and Sequence Analysis of LIP Gene from Bacillus Licheniformis BL03 |
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Affiliation: | LIU Sheng-feng et al(Chongqing Entry-Exit Inspection and Quarantine Bureau of China,Chongqing 400020) |
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Abstract: | [Objective] To clone Bacillus licheniformis BL03 gene LIP,and analyze the sequence.[Method] The LIP gene encoding lipase from B.licheniformis BL03 was amplified by PCR and cloned to the vector pMD19-T.[Result] Nucleotide sequence analysis of the LIP gene revealed that the coding region was formed of one complete ORF including 615 base pairs.The cloned gene was 99.67% similar with B.licheniformis LIP gene(GeneID:3098655) and the putative amino acid sequence was 99.02% similar respectively.[Conclusion] The cloned gene laid a foundation for the heterologous expression and relevant researches. |
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Keywords: | Bacillus licheniformis LIP gene Cloning Sequence analysis |
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