| 一株产蛋白酶地衣芽孢杆菌的分离与鉴定 |
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| 引用本文： | 石笛,江洁芳,钟瑜,黄琼. 一株产蛋白酶地衣芽孢杆菌的分离与鉴定[J]. 安徽农业科学, 2012, 40(29): 14195-14196,14202 |
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| 作者姓名： | 石笛 江洁芳 钟瑜 黄琼 |
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| 作者单位： | 广州市微生物研究所,广东广州,510663;广州市微生物研究所,广东广州,510663;广州市微生物研究所,广东广州,510663;广州市微生物研究所,广东广州,510663 |
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| 摘 要： | [目的]对产蛋白酶的地衣芽孢杆菌进行分离与鉴定。[方法]从饲料样品中筛选出产蛋白酶的菌株,对其进行形态和生理生化特征鉴定,并构建该菌株的16S rDNA系统发育树。[结果]分离到的菌株GW201205与地衣芽孢杆菌(Bacillus licheniformis)的生理生化特征相同,且其16S rDNA序列与B.licheniformis相似性最高。[结论]可将试验分离到的菌株GW201205初步鉴定为B.licheniformis。
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| 关 键 词： | 蛋白酶 地衣芽孢杆菌 分离 鉴定 |
Isolation and Identification of a Bacillus licheniformis with Protease Activity |
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| Affiliation: | SHI Di et al(Guangzhou Microbe Research Institute,Guangzhou,Guangdong 510663) |
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| Abstract: | [Objective] To isolate and identify a Bacillus licheniformis with protease activity.[Method] A strain with protease activity was isolated from the feed samples,and then its morphological and biochemical characteristics were identified,a 16S rDNA phylogenetic tree of this strain was constructed.[Result] The isolated strain GW201205 showed similar biochemical characteristics and 16S rDNA to Bacillus licheniformis.[Conclusion] The isolated strain GW201205 is preliminarily identified to be Bacillus licheniformis. |
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| Keywords: | Protease Bacillus licheniformis Isolation Identification |
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