应用Taqman荧光定量PCR快速检测宠物食品中的沙门氏菌

[目的]建立快速检测宠物食品中沙门氏菌(Salmonella)的荧光定量PCR方法。[方法]根据Genbank中登录的沙门氏菌吸附和侵袭上皮细胞表面蛋白invA基因序列设计合成引物和探针,检测该方法的特异性和敏感性。[结果]该方法具有良好的特异性,对沙门氏菌的检测灵敏度可达到17 CFU/反应(25μl/反应)。应用该方法检测人工污染宠物食品,样品经18 h增菌后,结果均为阳性。[结论]该研究建立的Taqman荧光定量PCR方法可以快速、准确地检测宠物食品中的沙门氏菌。

Taqman PCR-based Methods for Rapid Detection of Salmonella in Pet Food

[Objective] To establish a Taqman real-time PCR for detection of Salmonella in pet food.[Method] A pair of primers and a probe were designed based on published nucleotide sequence of invA gene encoding the invasion protein of Salmonella enterica.[Result] The assay detects Salmonella specifically.The detection limit of the real-time PCR was 17 CFU/test(25 μl/test) for the positive strain.This method was effective to detect artificially contaminated pet food.[Conclusion] The results showed that Taqman PCR assay was rapid and accure for detection of Salmonella from infected pet food.