富硒灵芝的优化培养

[目的]筛选富硒灵芝菌丝体的最佳培养方法。[方法]采用单因素试验和正交试验研究发酵培养基的碳源、氮源以及发酵条件对富硒灵芝菌丝体干重、总硒含量及有机硒含量的影响。[结果]在亚硒酸钠含量为0.01%的条件下,优化培养的条件为:葡萄糖含量4.0%,马铃薯浸出液含量20%,蛋白胨含量1%,KH2PO4含量0.3%,MgSO4含量0.15%,在pH7.0、接种量为10%、培养温度为28℃和摇床转速为180 r/min的条件下培养,富硒灵芝的菌丝干重为15.28 mg/ml,有机硒的含量为4 115.36μg/g。[结论]试验确定了富硒灵芝发酵培养的最佳方法,为富硒灵芝的开发利用提供了依据。

Optimization of Culture Conditions of Se-enriched Ganoderma lucidum

[Objective] To find the optimal culture conditions of Se-enriched Ganoderma lucidum mycelium.[Methods] The effects of carbon source,nitrogen source and fermentation conditions on the dry weight,total selenium and organic selenium content of Se-enriched G.lucidum mycelium were studied by using single factor test and orthogonal test.[Result] Under conditions of 0.01% sodium selenite content,glucose content 4%,potato extracting 20%,peptone content 1%,KH2PO4 0.3%,MgSO4 0.15%,pH=7,inoculation amount 10%,culture temperature 28 ℃,and shaking speed 180 r/min,the dry weight of Se-enriched G.lucidum mycelium was 1.528 g/100 ml,and the organic selenium content was 4 115.36 μg/g.[Conclusion] The optimal culture conditions of Se-enriched G.lucidum were determined,which could provide basis for development and utilization of Se-enriched G.lucidum.