不同球孢白僵菌菌株生物学特性与其对家蚕致病力的关系

[目的]探讨球孢白僵菌菌株的生物学特性与其对家蚕致病力的关系,为家蚕白僵病的防治提供科学依据。[方法]从我国各地收集6个白僵菌杀虫剂,并从广西养蚕区采集3个僵蚕样品,对从中分离纯化的菌株进行形态观察和分子鉴定,同时对家蚕进行致病力测定,比较各菌株菌落直径、产孢量、胞外蛋白酶产生水平等生物学特征指标的差异。[结果]获得的9个分离菌株均为球孢白僵菌(Beauveria bassiana Vuillemin);各菌株对家蚕都有较强致病力,且致病力有一定差异;各菌株菌落生长直径、产孢量及胞外蛋白酶活性均存在较大差异,并与其对家蚕的致病力之间存在相关关系。[结论]各菌株产孢量和胞外蛋白酶活性与其对家蚕的致病力之间存在显著正相关。

Relationship between Biological Characteristics of Beauveria bassiana(Bals.) Vuill and Pathogenicity to Bombyx mori L.

[Objective] This study was to investigate the relationship between biological characteristics of Beauveria bassiana(Bals.) Vuill and pathogenicity of Bombyx mori L.,with the aim to provide scientific basis for the control of white muscardine in Bombyx mori L..[Method] The strains isolated and purified from the 6 Beauveria bassiana biocontrol agents from all over the country and the 3 white muscardine silkworms collected from Guangxi provincial silkworm rearing areas were identified by the morphological observation and molecular biology technology.The pathogenicity of silkworms to B.bassaina was determined,and the biological characteristics such as growth diameter,spore production amount and the extracellular protease activity of the different B.bassiana strains were compared.[Result] The isolated 9 strains were all B.bassaina(Bals.) Vuillemin,and all strains had high pathogenicity to silkworm,but with different pathogenicities.The growth diameter,spore production amount and extracellular protease activity of different B.bassiana strains were also different,and showed correlation with the patheogenicity to silkworms.[Conclusion] B.bassiana spores production amount and exocellular protease activity had significant positive correlation with their pathogenicity to silkworm.