苎麻茎皮表达序列标签(ESTs)分析

以生长中期的苎麻Boehmeria nivea (L.) Gaud]茎皮为材料,构建库容量为2.2×105pfu/mL的cDNA文库并随机挑选部分克隆进行测序和生物信息学初步分析,得到275条有效序列.通过与NCBI等数据库比对分析,获得已知功能基因或具推测功能基因的EST91条,已知EST37条,新的EST147条,具已知或推测功能的ESTs大致分为9类,其中与蛋白质合成有关的ESTs所占比例最大.cDNA文库的构建和ESTs分析为苎麻功能基因组学研究提供了一条方便快捷的途径.

nalysis of Expressed Sequense Tags(ESTs) of the Ramie[Boehmeria nivea(L.)Gaud] Barks

A cDNA library(2.2×105pfu/mL) was constructed from the ramieBoehmeria nivea(L.) Gaud] barks harvested at the mid-growth stage. The sequencing of cDNA clones and the analysis of expressed sequence tags (EST) were reported, and 275 valid ESTs were generated, including 91 ESTs with known or putative function, 37 ESTs with known function, and 147 novel ESTs, which were identified by BlastN searches against the NCBI non-redundant nucleotide databases. These ESTs were classified into 9 different categories, the highest proportion of which was related to protein synthesis. Further studies on the ESTs in the library may provide a rapid and effective approach for future study of functional genomics of ramie Boehmeria nivea(L.)Gaud].