抗氧化酶对棕色棉色泽的影响

 以棕色棉和白色棉为材料，测定发育期种皮与纤维中抗氧化酶及色素合成相关物质含量的变化，初步探讨抗氧化酶对棕色棉色泽的影响。结果表明：纤维次生壁沉积期（25~45 DPA），棕色棉种皮颜色显著加深；次生壁沉积后期（35~45 DPA），棕色棉纤维颜色加深明显。花后28~35 d，浅棕ANL-2种皮缩合单宁和总酚含量下降，棕色素积累；花后35~42 d，深棕ANL-1纤维缩合单宁含量降低，总酚含量上升，纤维颜色变化最深。花后28~35 d，浅棕ANL 2种皮POD活性下降；花后35~42 d，棕色棉纤维POD活性降低；SOD与CAT对棕色棉色泽影响不大；POD在次生壁沉积后期（35~45 DPA）参与棕色素的形成。

Effect of Antioxidases on Color of Brown Cotton

Brown and white cotton cultivars were used as materials, the change of the content of related substances to pigment synthesis and the activities of antioxidases both in the fiber and the seed capsule during the development were investigated, and the effect of antioxidases on color of brown cotton was studied. The results indicated that the color of the seed capsule darkened apparently during the secondary cell wall thickening period（25～45 DPA）;the color of the fiber darkened obviously during the secondary cell wall thickening period（35～45 DPA）.The content of condensed tannin and the phenol in the seed capsule of light brown ANL-2 decreased and pigments accumulated from 28 to 35 DPA; the content of condensed tannin in the fiber of dark brown ANL-1 decreased ,while the content of the phenol increased ,and the color of the fiber became darkest from 35 to 42 DPA. The activity of POD in the seed capsule of light brown ANL-2 from 28 to 35 DPA decreased, and the activity of POD in the fiber of brown cotton decreased from 35 to 42 DPA; the effect of SOD and CAT on color of brown cotton was small. POD participated in the formation of pigments during the secondary cell wall thickening period（35～45 DPA）.