| 基于改进CTAB法提取空间诱变柱花草总DNA研究 |
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| 引用本文： | 严琳玲,赵琼玲,白昌军,刘国道. 基于改进CTAB法提取空间诱变柱花草总DNA研究[J]. 安徽农业科学, 2009, 37(8): 3465-3467 |
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| 作者姓名： | 严琳玲 赵琼玲 白昌军 刘国道 |
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| 作者单位： | 海南大学儋州校区农学院,海南儋州,571737;中国热带农业科学院热带作物品种资源研究所,海南儋州,573717;中国热带农业科学院热带作物品种资源研究所,海南儋州,573717 |
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| 基金项目： | 农业部“973”项目(2007CB108903) |
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| 摘 要： | [目的]用改进CTAB法提取空间诱变柱花草的总DNA。[方法]以柱花草幼苗叶片为材料，采用改良CTAB法提取柱花草总DNA。[结果]所提的DNA凝胶条带清晰，无拖尾现象，浓度在406．6～857．3ng／μl，0D260／DD230检测值在1．96—2．18，OD260／0D280检测值在1．76—1．98。所提DNA适用于ISSR-PCR。[结论]该方法具有简便、快速、高效的特点。
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| 关 键 词： | 柱花草 空间诱变 CTAB DNA ISSR-PCR |
Extraction of DNA from the Space Mutation in Stylo by Improved CTAB Method |
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| YAN Lin-ling et al. Extraction of DNA from the Space Mutation in Stylo by Improved CTAB Method[J]. Journal of Anhui Agricultural Sciences, 2009, 37(8): 3465-3467 |
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| Authors: | YAN Lin-ling et al |
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| Affiliation: | YAN Lin-ling et al(College of Agronomy,Hainan University,Danzhou,Hainan 571737) |
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| Abstract: | [ Objective ] The research aimed to extract the genomie DNA of Stylosanthes spp. by the improved method of CTAB-DNA isolation. [ Method] The improved method of CTAB-DNA isolation was used to extract genomic DNA from the seedling leaves of the space mutation in Stylosanthes spp, [ Result] High quality total DNA was isolated, and the concentration was 406.6 -857.3 ng/μl. The values of OD260/OD230 of each sample were between 1.96 and 2.18, the values of OD260/OD280 were between 1.76 and 1.98. The DNA extracted by CTAB can be used for ISSR-PCR. [ Conclusion] The improved method of CTAB-DNA isolation had characteristics of simple, rapid and effective. |
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| Keywords: | Stylosanthes spp. Space mutation CTAB DNA ISSR-PCR |
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