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3M纸片检测大肠菌群法与国标快速检测法的比较研究
引用本文:范远景,潘丙南,陈伟,韩明忠,程仁剑,李艳. 3M纸片检测大肠菌群法与国标快速检测法的比较研究[J]. 安徽农业科学, 2009, 37(1)
作者姓名:范远景  潘丙南  陈伟  韩明忠  程仁剑  李艳
作者单位:1. 合肥工业大学生物与食品工程学院,安徽合肥,230039
2. 安徽凯利粮油食品公司,安徽合肥,231600
基金项目:安徽省科技攻关计划重大科技专项基金 
摘    要:[目的]比较用3M纸片法与用快速检测国标法(MEFH)对不同样品大肠菌群的检测结果。[方法]以花生、小米、玉米、大米和花生油为检测样品,以Aliz-gal和MUGal肉汤为培养基,设大肠菌群污染的自然状态未知浓度和模拟培养已知浓度的两种处理,进行3M纸片法与MEFH法对大肠菌群的检测结果对比试验。[结果]3M法与MEFH中MPN法检测自然状态的5种样品的大肠菌群结果比较符合率为90%,3M纸片法和MEFH中的平板法检测结果无明显差异。3M纸片法未出现假阳性现象,证明了3M纸片法有很强的选择性。[结论]3M纸片法操作简便,适合企业现场食品卫生检测。

关 键 词:大肠菌群  3M纸片法  MPN法  平板法

Comparative Study on Detection Methods of Coliform Group by Using 3M Petrifilm and E.coli Count Plate with MEFH-RDC
FAN Yuan-jing et al. Comparative Study on Detection Methods of Coliform Group by Using 3M Petrifilm and E.coli Count Plate with MEFH-RDC[J]. Journal of Anhui Agricultural Sciences, 2009, 37(1)
Authors:FAN Yuan-jing et al
Affiliation:FAN Yuan-jing et al (Institute of Biological , Food Engineering,Hefei University of Technology,Hefei,Anhui 230039)
Abstract:[Objective] The study aimed to compare the results of detecting the coliform group by the method of 3M petrifilm and E.coli count plate and by microbiological examination of food hygiene-rapid detection of coliform bacteria(MEFH-RDC) method.[Method] With peanut,millet,corn,rice and peanut oil as the tested samples and the Aliz-gal and MUGal broth as the medium,2 treatments of nature with unknown concn.and simulation with known concn.for coliform bacteria group were set up to conduct the comparative test on ...
Keywords:Coliforms group  Method of 3M petrifilm and E.coli count plate  MPN method  Spread plate method  
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