RAPD-based Analysis of Genetic Diversity and Selection of Lingonberry (<Emphasis Type="Italic">Vaccinium vitis-idaea</Emphasis> L.) Material for <Emphasis Type="Italic">ex situ</Emphasis> Conservation |
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Authors: | Email author" target="_blank">L?Garkava-GustavssonEmail author HA?Persson H?Nybom K?Rumpunen BA?Gustavsson IV?Bartish |
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Institution: | 1.Balsg?rd–Department of Crop Science,Swedish University of Agricultural Sciences,Kristianstad,Sweden;2.Department of Phanerogamic Botany,Swedish Museum of Natural History,Stockholm,Sweden |
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Abstract: | Random amplified polymorphic DNA markers were used to assess relatedness and genetic diversity for 15 lingonberry (Vaccinium vitis-idaea) populations. Seven primers yielding 59 polymorphic bands were used to analyse 13 populations, representing ssp. vitis-idaea from Sweden, Finland, Norway, Estonia and Russia, and two populations, representing ssp. minus from Japan and Canada. A cluster analysis and a multidimensional scaling analysis (MDS) showed similar phenetic patterns
among populations, with a pronounced geographic grouping in most cases. Significant correlations were obtained between geographic
and genetic distances for the entire set of populations as well as for the 13 ssp. vitis-idaea populations. Mean within-population diversity was 0.206 when estimated with Lynch and Milligan's index, and 0.431 when estimated
with Shannon's index, which is in agreement with the mixed mating system reported for lingonberry. Within-population variability
accounted for 68.6% of the total variance when all populations were included, and for 78.8% when only populations of ssp.
vitis-idaea were analysed. Two different approaches were applied to the selection of plant material for a potential gene bank: (1) a
hierarchical sampling strategy based on a cluster analysis and (2) the Maximum genetic diversity program, developed for the
establishment of core collections. Random sampling was undertaken for comparisons with the selected data sets. The most diverse
and representative set of lingonberry specimens was obtained when samples were selected with the Maximum diversity program. |
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Keywords: | Genetic relatedness Germplasm collection Molecular markers Population genetics |
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