| 金合欢组织培养和快速繁殖的研究 |
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| 引用本文： | 翟建中,顾梅俏,程荣昌,张燕,刘小军.金合欢组织培养和快速繁殖的研究[J].园艺学报,2001,28(2):149-152. |
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| 作者姓名： | 翟建中 顾梅俏 程荣昌 张燕 刘小军 |
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| 作者单位： | ( 上海市花卉育种中心, 上海201615 ; 上海松江花桥现代农业公司, 上海201601) |
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| 摘 要： | 采用金合欢(Acacia dealbata) 大树顶芽、幼苗茎尖、种子小苗上胚轴和幼叶作外植体进行组培和快繁研究。结果表明: 用MS + 6-BA 6 mg/L + NAA 0. 1 mg/L + NaH2PO4 30 mg/L 可提高愈伤组织的出芽率, 有利于扩大繁殖系数, 而用MS + 6-BA 2 mg/L + NAA 0. 1 mg/L + NaH2PO4 30 mg/L 可以增加发枝率。珍珠岩+ 泥炭(1∶1) 基质可促进小苗后期生长,加速成苗。
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| 关 键 词： | 金合欢 组织培养 快速繁殖 炼苗 |
| 文章编号： | 0513-353X(2001)02-0149-04 |
| 修稿时间： | 2000年11月7日 |
Tissue Culture and Rapid
Propagation of Acacia dealbata |
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| Zhai Jianzhong,Gu Meiqiao,Chen Rongchang,Zhang Yan,Liu Xiaojun.Tissue Culture and Rapid
Propagation of Acacia dealbata[J].Acta Horticulturae Sinica,2001,28(2):149-152. |
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| Authors: | Zhai Jianzhong Gu Meiqiao Chen Rongchang Zhang Yan Liu Xiaojun |
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| Institution: | ( Shanghai Flower Breeding Center , Shanghai 201615; Shanghai Songjiang Rose Bridge Modern Agriculture Company Limited , Shanghai 201601) |
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| Abstract: | Acacia dealbata is a famous kind of economical an d oremental trees in Australia.In this test，shoot tips taken from big trees and seedlings，little leaves from young plants，epicotyls from seeds were collected as explants that were studied on tissue culture and rapid propagation.The resul ts showed that all four kinds of explants have been induced callus formation.Bud s and shoots can be induced on callus except little leaf's callus in morphogensis.The medium MS+6-BA 6 mg/L+NAA 0.1 mg/L+NaH2PO4 30 mg/L may increase sprouting bud rate from callus.Ho wever，the medium MS+6-BA 2 mg/L+NAA 0.1 mg/L+NaH2 PO4 30 mg/L may increase shoot growing rate.Plantlets from test tubes g rew better on the growth medium perlite+peat(1∶1)than on other components in acclimatization stage. |
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| Keywords: | Acacia dealbata Tissue culture Rapid propagation Acclimatization |
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