Multi-centered investigation of a point-of-care NT-proBNP ELISA assay to detect moderate to severe occult (pre-clinical) feline heart disease in cats referred for cardiac evaluation |
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| Institution: | 1. Department of Clinical Studies – Philadelphia, School of Veterinary Medicine, University of Pennsylvania, 3900 Delancey St, Philadelphia, PA 19104, USA;2. Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA;3. Department of Clinical Sciences, Cummings School of Veterinary Medicine at Tufts University, 200 Westboro Rd, North Grafton, MA 01536, USA;4. Michigan Veterinary Specialists, 29080 Inkster Rd, Southfield, MI 48034, USA;5. Department of Medical Sciences, University of Wisconsin, 2015 Linden Dr, Madison, WI 53706, USA;6. The Animal Medical Center, 510 East 62nd St, New York, NY 10065, USA;1. Clinical Science and Services, Royal Veterinary College, Hawkshead Lane, North Mymms, Hertfordshire, AL9 7TA, United Kingdom;2. Langford Veterinary Services, University of Bristol, Langford House, Lower Langford, Bristol, BS40 5DU, United Kingdom;1. The Animal Medical Center, 510 East 62nd Street, New York, NY 10065, USA;2. Department of Veterinary Clinical Sciences, The Ohio State University, 601 Vernon L Tharp Street, Columbus, OH 43210, USA;1. Department of Small Animal Clinical Sciences, Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, USA;2. Department of Companion Animals, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE, Canada;1. Department of Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, Box 7054, Uppsala, Sweden;2. The Royal Veterinary College, Department of Clinical Science and Services, Hawkshead Lane, Hatfield AL9 7TA, United Kingdom;3. Clinic of Small Animal Medicine, LMU University, Veterinaerstr. 13, 80539 Munich, Germany;1. Department of Veterinary Clinical and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg, Denmark;2. Laboratory of Applied Statistics, Department of Mathematical Sciences, Faculty of Sciences, University of Copenhagen, Frederiksberg, Denmark |
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| Abstract: | ObjectiveTo prospectively evaluate the diagnostic accuracy of a point-of-care (POC) N-terminal pro-B-type natriuretic peptide (NT-proBNP) ELISA to assess the likelihood of moderate to severe occult heart disease (OcHD) in a clinical population of cats suspected to have heart disease.AnimalsOne hundred and forty-six asymptomatic client-owned cats with a heart murmur, gallop rhythm, arrhythmia, or cardiomegaly.MethodsPhysical examination, blood pressure measurement and echocardiography were performed prospectively. Point-of-care ELISA was visually assessed as either positive or negative by a reader blinded to the echocardiographic results.ResultsForty-three healthy cats, 50 mild OcHD, 31 moderate OcHD, 6 severe OcHD, and 16 cats equivocal for OcHD were examined. Cats with OcHD included 65 with hypertrophic cardiomyopathy, 6 with restrictive or unclassified cardiomyopathy, 1 with arrhythmogenic right ventricular cardiomyopathy, and 15 with non-cardiomyopathic forms of heart disease. Point-of-care ELISA differentiated cats with moderate or severe OcHD with sensitivity/specificity of 83.8%/82.6% and overall accuracy of 82.9%. Positive POC ELISA increased likelihood of moderate or severe OcHD by a factor of 4.8 vs. those that tested negative. Point-of-care ELISA differentiated cats with moderate or severe cardiomyopathic OcHD with sensitivity/specificity of 88.6%/81.3% and overall accuracy of 83.2%.ConclusionIn a select sample of cats referred for cardiac evaluation, positive POC NT-proBNP ELISA increases likelihood of moderate to severe OcHD while negative POC NT-proBNP ELISA result excludes moderate to severe OcHD. |
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| Keywords: | Blood tests BNP Natriuretic peptides Cardiomyopathy |
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