| 植物体内MDA不同提取液与显色液反应条件的比较 |
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| 引用本文： | 刘少霞,陈贵,秦萍,曹冬煦,马冬伍. 植物体内MDA不同提取液与显色液反应条件的比较[J]. 安徽农业科学, 2007, 35(10): 2829-2829,2832. DOI: 10.3969/j.issn.0517-6611.2007.10.002 |
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| 作者姓名： | 刘少霞 陈贵 秦萍 曹冬煦 马冬伍 |
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| 作者单位： | 沈阳农业大学生物科学技术学院,辽宁沈阳,110161;辽宁省沈阳市沈北新区疫病预防控制中心,辽宁沈阳,110121 |
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| 摘 要： | 用50 mmol/L磷酸缓冲液pH值7.8、去离子水、5%三氯乙酸3种提取液分别提取小苍兰叶片中的MDA,分别与5%三氯乙酸+0.5%TBA、1 mol/L HCl+0.5%TBA、30%乙酸+0.5%TBA 3种显色液进行交差显色试验.结果表明:用去离子水提取植物体内的MDA效果较好,用30%乙酸+0.5%TBA与MDA显色,反应产物在特定波长(532 nm)处有明显吸收峰值.
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| 关 键 词： | MDA 提取液 显色液 植物 |
| 文章编号： | 0517-6611（2007）10-02829-01 |
| 收稿时间： | 2007-01-10 |
| 修稿时间： | 2007-01-10 |
Comparisons on Reaction Conditions of Different Extraction Solvents for Plant Endogenous MDA and Chromogenic Reagent |
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| LIU Shao-xia ,et al. Comparisons on Reaction Conditions of Different Extraction Solvents for Plant Endogenous MDA and Chromogenic Reagent[J]. Journal of Anhui Agricultural Sciences, 2007, 35(10): 2829-2829,2832. DOI: 10.3969/j.issn.0517-6611.2007.10.002 |
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| Authors: | LIU Shao-xia et al |
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| Affiliation: | College of Biological Science and Technology, Shenyang Agricultural University, Shenyang, Liaoning 110161 |
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| Abstract: | Three extraction solvents of 50 mmol/L phosphate butler at pH 7.8,deionized water,5% TCA were used to extract MDA in leaves of Freesia refracta respectively.The MDA extracted by three extraction solvents were reacted with three chromogenic reagents of 5%TCA 0.5%TBA,1 mol/L HCl 0.5%TBA,30%HAC 0.5%TBA,respectively.The results showed that deionized water had better effect of extracting plant endogenous MDA and its reactions products with chromogenic reagent of 30%TCA 0.5%TBA had a remarkable absorption peak at the wavelength of 532 nm. |
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| Keywords: | MDA Extraction solvent Chromogenic reagent Plant |
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