| 牛副结核胶体金免疫层析试纸条的研制 |
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| 引用本文： | 乔榛,张丽娟,秦建华,赵月兰,左玉柱. 牛副结核胶体金免疫层析试纸条的研制[J]. 畜牧与兽医, 2010, 42(1) |
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| 作者姓名： | 乔榛 张丽娟 秦建华 赵月兰 左玉柱 |
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| 作者单位： | 1. 河北农业大学动物科技学院,河北,保定,071001
2. 河北农业大学中兽医学院,河北,定洲,073000 |
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| 基金项目： | 国家科技支撑计划，河北省重大技术创新专项计划 |
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| 摘 要： | 将提纯的牛副结核分支杆菌重组蛋白MAP0862-2154c作为硝酸纤维素膜检测线的包被抗原,兔抗羊IgG作为硝酸纤维素膜质控线的包被抗体,金标羊抗牛IgG点喷到玻璃纤维素膜上,制成用于检测牛副结核抗体胶体金免疫层析试纸条。用牛的副结核标准阳性血清与检测试纸条反应,在检测线处出现红色反应带,而滴加阴性血清的试纸条检测线处未出现反应条带,上述2种血清在质控线处均出现红色反应条带;试纸条可检出牛副结核抗体的最低抗原包被浓度为400μg/mL;试纸条不与牛结核病、牛布鲁氏菌病的阳性血清发生反应;试纸条37℃保存9d后的检验结果与4℃保存的检验结果相同。所制备的试纸条具有灵敏、特异的优点,稳定性良好;10min左右即出结果,操作简便,可用于临床诊断。
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| 关 键 词： | 牛副结核 胶体金 免疫层析 试纸条 |
Development of collcidal gold immunochromatographic test strip for detection of bovine paratuberculosis |
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| QIAO Zhen,ZHANG Li-juan,QIN Jian-hua,ZHAO Yue-lan,ZUO Yu-zhu. Development of collcidal gold immunochromatographic test strip for detection of bovine paratuberculosis[J]. Animal Husbandry & Veterinary Medicine, 2010, 42(1) |
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| Authors: | QIAO Zhen ZHANG Li-juan QIN Jian-hua ZHAO Yue-lan ZUO Yu-zhu |
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| Affiliation: | QIAO Zhen1,ZHANG Li-juan1,QIN Jian-hua1,ZHAO Yue-lan2,ZUO Yu-zhu1 (1.College of Animal Science , Technology,Hebei Agriculture University,Baoding 071000,China,2.College of Chinese Traditional Veterinary Science,Dingzhou 073000,China) |
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| Abstract: | The purified recombinant protein of Mycobacterium paratuberculosis, MAP0862-2154c, was used as coated antigen at the detected line position of nitrocellulose membrane, while rabbit anti-sheep IgG was used as antibody at the control line position of nitrocellulose membrane, and colloidal gold labeled goat anti-bovine IgG was sprayed on glass cellulose membrane, thus to prepare the colloidal gold immunochromatograpic strip. A red reaction strip at the test line position appeared only when the standard positiv... |
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| Keywords: | bovine paratuberculosis colloidal gold immunochromatography test strip |
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