绢蒿水煎剂对小鼠免疫功能的影响

为了探讨新疆石河子南山地区牧草绢蒿对正常小鼠和环磷酰胺所致免疫低下小鼠的免疫调节作用,采用脾脏系数测定法、脾脏淋巴细胞ANAE染色法、姬姆萨-瑞特氏染色法、MTT法、抗支原体抗体ELISA法及大肠杆菌攻毒试验分别对小鼠脾脏指数、T淋巴细胞数量、巨噬细胞吞噬活性、脾脏淋巴细胞增殖、抗体分泌功能及抗致病性大肠杆菌感染能力等指标进行研究。结果表明,高、低浓度的绢蒿水煎剂均能不同程度提高免疫低下小鼠和正常小鼠的T淋巴细胞数量及腹腔巨噬细胞吞噬功能;高、低浓度的绢蒿水煎剂均能不同程度提高正常小鼠的脾脏淋巴细胞增殖能力;低浓度的绢蒿水煎剂能提高正常小鼠对大肠杆菌的抗感染能力;高浓度的绢蒿水煎剂能下调免疫低下小鼠和正常小鼠的脾脏指数,下调正常小鼠的血清抗体效价。提示,绢蒿水煎剂对免疫低下小鼠有一定的增强免疫作用,对正常小鼠具有双向免疫调节作用。本研究结果为进一步开发绢蒿免疫调节剂提供了依据。

Effect of Seriphidium Decoction on Immunologic Function of Mice

To investigate the effect of Seriphidium (Bess) Poljakin the Nanshan area, Shihezi, Xinjiang province on immunologic function of the normal and cyclophosphamide-induced hypoimmunitymice mice, the spleen index was studied by the organ coefficient method; the number of T lymphocyte of the spleen was studied by ANAE method; the phagocytic function of the peritoneal macrophages was studied by R-J stain method; the proliferation of spleen lymphocyte was studied by the MTT method; the antibody secretion was studied by ELISA method of the resistance of mycoplasma antibodies; the anti infection ability of the pathogenic Escherichia coli was studied by the Escherichia coli tapping poison test method. The results showed that Seriphidium decoction could improve positive rates of ANAE of T lymphocyte and phagocytic rates of the abdominal macrophage cell in the normal and cyclophosphamide-induced hypoimmunity mice; Seriphidium decoction could improve proliferative abilities of spleen lymphocyte on the normal mice; low dose of Seriphidium decoction could improve resistance to infection of Escherichia coli; high dose of Seriphidium decocion could decrease serum antibody titer and spleen index in the normal mice. In short, Seriphidium decoction could enhance immunological fuction on the cyclophosphamide-induced hypoimmunity mice; and had a two-way effect on the immunologic function of the normal mice. The results of the study provided the basis for the further development of immune regulator of Seriphidium.