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Effect of bovine somatotropin and food deprivation on β-adrenergic and A1 adenosine receptor binding in adipose tissue of lactating cows |
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| Authors: | K. L. Houseknecht D. E. Bauman G. B. Carey H. J. Mersmann |
| Affiliation: | a Department of Animal Science, Cornell University, Ithaca, NY, USA b Department of Animal and Nutritional Sciences, University of New Hampshire, Durham, NH, USA c USDA/ARS, Children's Nutrition Research Center, Houston, TX, USA |
| Abstract: | Lactating Holstein cows were used to assess the effect of bovine somatotropin (bST; n = 8) and fasting (FAST; n = 4) on ligand binding to β-adrenergic (BAR) and TYP e-1 adenosine (A1R) receptors in adipose tissue. Cows received exogenous bST (sometribove; 40 mg/d) or no hormone (control) for 4 d in a single-reversal design with a 7-d interval between treatment periods. Subcutaneous adipose tissue biopsies were taken on day 4 of each treatment. Eight d after the bST regimen, 4 cows were fasted for 3 d and adipose biopsies were taken. Ligand binding was quantified with a postnuclear, total adipose tissue membrane preparation (100,000 × g pellet). Binding to BAR and A1R was assessed with the antagonists [125I]iodocyanopindolol (ICP) and [3H]8-cyclopentyl-1,3-dipropylxanthine (DCPCX), respectively. The binding affinity (Kd) of BAR for ICP was not affected by bST but was enhanced by FAST; maximal binding (Bmax) was increased with bST treatment (P < 0.06) and reduced by FAST (61%, P < 0.01). Kd values for DCPCX binding to A1R were not changed by bST or FAST. bST did not affect Bmax for A1R; however, FAST reduced the Bmax by 38%. Data highlight the differential regulation of BAR and A1R by bST and FAST. |
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