蚕丝丝胶蛋白提取方法的研究

应用冰冻法和透析法研究了柞蚕和家蚕丝胶的提取方法。结果表明,提取柞蚕丝胶蛋白的最佳工艺是:蚕丝脱胶液pH值为7,在-20℃冰冻13h,回收率可达58%;提取家蚕丝胶的最佳工艺是:蚕丝脱胶液pH值为7,在-20℃冰冻11h,回收率可达75%;应用透析法提取丝胶蛋白,在所用透析膜为8000～20000D的情况下,柞蚕丝胶蛋白的回收率为20.0%,而家蚕丝胶蛋白的回收率可达81.5%。

Study on Methods of Silk Sericin Recovery

The different distilling methods freeze- method and dialyze- method of tussah and silkworm sericin were studied. The optimum technology of recovery sericin of tussah was that liquid coming unglued of silk was frozen under -20 ℃ for 13 h in pH=7. The rate of recovery was 58% left and right sides. It was the best that liquid coming unglued of silkworm was frozen under -20 ℃ for 11h in pH=7, The rate of recovery can reach 75% left and right sides. About the dialyze-method, under the circumstances of 8 000~20 000 D for the dialyzable membrane, the receive ratio of tussah was 20.0%, and the receive ratio of silkworm can reach 81.5%.