泰山赤鳞鱼酪氨酸酶的活性和表达差异(英文)

目的]对泰山赤鳞鱼中酪氨酸酶的活性和表达差异进行研究。方法]以泰山赤鳞鱼为材料,运用聚丙烯酰胺凝胶电泳与生化染色技术、分光光度技术和酶组织化学技术对赤鳞鱼雌、雄个体9种组织中的酪氨酸酶进行了分析研究。结果]酪氨酸酶在雌、雄赤鳞鱼的肝胰脏、肠和脾组织中均有存在;同时在雄性赤鳞鱼的胆中也有分布。赤鳞鱼各组织中酪氨酸酶的活性大小存在较大差异。其中,雌雄性赤鳞鱼的脾脏中活性最强,但其活性低于雌性鱼脾中的酪氨酸酶活性。酶组织化学结果显示赤鳞鱼的脾脏、肠、肝胰脏和胆中都存在较强的酪氨酸酶阳性信号,而且脾中的阳性信号最强。结论]该研究是关于泰山赤鳞鱼酪氨酸酶组织定位研究的首次报道,为进一步了解赤鳞鱼酪氨酸酶的功能提供了理论依据。

Research on Activity and Expression of Tyrosinase in Varicorhinus macrolepis

Objective] This study aimed to analyze tyrosinase activity and its expression in Varicorhinus macrolepis. Method] V. macrolepis was used as experimental material for the analysis and research of tyrosinase in nine kinds of organs and tissues of male and female V. macrolepis individuals by using polyacrylamide gel electrophoresis and biochemical staining method, spectrophotometry and enzyme histochemical technology. Result] Tyrosinase exists in the liver and pancreas, intestine and spleen of female and male V. macrolepis and in the gallbladder of male V. macrolepis. Tyrosinase activities in various tissues of V. macrolepis varied largely. Specifically, tyrosinase activities in the spleen was the maximum, which was higher in female V. macrolepis than in males. According to the enzyme histochemistry results, strong positive signals of tyrosinase existed in the spleen, intestine, liver and pancreas and gallbladder of V. macrolepis, which was the strongest in the spleen. Conclusion] In this paper, research on tissue localization of tyrosinase in V. macrolepis had been first reported, which provided theoretical basis for further exploring the functions of tyrosinase in V. macrolepis.