| 柑桔黄龙病的直接荧光诊断法 |
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| 引用本文： | 吴世盘. 柑桔黄龙病的直接荧光诊断法[J]. 华南农业大学学报, 1987, 0(2) |
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| 作者姓名： | 吴世盘 |
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| 作者单位： | 广东省柑桔黄龙病研究小组 |
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| 摘 要： | 试验证明,直接荧光诊断法是诊断柑桔黄龙病的一种快速、简便、准确的方法。 将健叶和黄龙病叶叶柄用保险刀片徒手切片,在透射式荧光显微镜下检查,可看到健叶叶柄的木质部导管细胞壁发黄色荧光,韧皮纤维细胞发绿色荧光,而病叶叶柄韧皮部中有1～多个鲜明的黄色或黄绿色荧光团块。这种特异性的荧光,在健叶柄韧皮部中是没有的,其它由病毒、类病毒、细菌、真菌等病原引起的病叶柄韧皮部中亦没有此种特异性荧光。
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| 关 键 词： | 诊断 荧光 菌原体 坏死 病毒 黄龙病 |
DIRECT FLUORESCENCE DETECTION METHOD FOR DIAGNOSING CITRUS YELLOW SHOOT DISEASE |
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| Wu Shihparn. DIRECT FLUORESCENCE DETECTION METHOD FOR DIAGNOSING CITRUS YELLOW SHOOT DISEASE[J]. JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY, 1987, 0(2) |
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| Authors: | Wu Shihparn |
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| Affiliation: | Citrus Yellow Shoot Study Group of Guangdong Province |
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| Abstract: | This article outlines a rapid simple and reliable method for diagnosing citrus yellow shoot (YS) it was named "direct fluorescence detection method" . Thin leaf section were prepared from fresh tissues of YS-lnfected and healthy citrus plants, when observed under a fluorescence microscope (Type, Olympus AH-FL-2), a yellow fluorescence was distinctly observed in the YS-infected phloem tissues and not in the phloem tissuses of healthy plants and of those infected with tristeza, exocortis, canker, scab, greasy spot, Mg and Za deficiency. |
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| Keywords: | diagnosis Fluorescence Mycoplasma necrosis virus yellow shoot |
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