| 多年生黑麦草组织培养与植株再生研究 |
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| 引用本文： | 张万军,王涛. 多年生黑麦草组织培养与植株再生研究[J]. 草地学报, 2003, 11(3): 219-222. DOI: 10.11733/j.issn.1007-0435.2003.03.006 |
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| 作者姓名： | 张万军 王涛 |
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| 作者单位： | 中国农业大学农业与生物技术国家重点实验室, 北京, 100094 |
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| 摘 要： | 对多年生黑麦草种子为外植体的植株再生过程进行系统研究,结果表明,在改良的MS培养基(MSM),以MS无机盐+9.9mg/L维生素B1+9.5mg/L维生素B6+4.5mg/L尼克酸+1mg/LCH+30g/L蔗糖+琼脂8g/L为基本成分,附加5mg/L2,4-D和0.05mg/LKT时,适合种子的愈伤组织诱导;继代培养基附加2mg/L2,4-D和0.1mg/LKT;分化培养基附加1mg/L2,4-D和1mg/LKT;生根培养基为无激素的MS培养基。完成植株再生约需12周,愈伤组织分化率为70%。
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| 关 键 词： | 生物技术 多年生黑麦草 组织培养 再生 |
| 文章编号： | 1007-0435(2003)03-0219-04 |
| 收稿时间： | 2002-12-10 |
| 修稿时间： | 2002-12-10 |
A Study on the Tissue Culture and Plant Regeneration of Perennial Ryegrass |
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| ZHANG Wan-jun,WANG Tao. A Study on the Tissue Culture and Plant Regeneration of Perennial Ryegrass[J]. Acta Agrestia Sinica, 2003, 11(3): 219-222. DOI: 10.11733/j.issn.1007-0435.2003.03.006 |
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| Authors: | ZHANG Wan-jun WANG Tao |
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| Affiliation: | National Laboratory for Agriculture and Biotechnology, China Agriculture University, Beijing, China 100094 |
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| Abstract: | A systemic study of plant regeneration of perennial ryegrass has been conducted.The result showed the modified MS medium (MSM) that composed of MS salt +9.9 mg/L vitamin B1+9.5 mg/L vitamin B6+4.5 mg/L nicotinic acid+1 mg/L hydrolyze casine+30 g/L sucrose+8 g/L agar as basic medium was suitable for inducing callus formation from ryegrass seeds when 5 mg/L 2,4-D and 0.05 mg/L KT added.The subculture medium containing 2 mg/L 2,4-D and 0.1 mg/L KT.The medium for callus differentiation into plantlets containing 1 mg/L 2,4-D and 1 mg/L KT.The medium for regenerated plantlets rooting was basic MS medium free of hormone.The whole period of ryegrass plant regeneration takes about 12 weeks.The differentiation ratio of calli reached 70%. |
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| Keywords: | Biotechnology Perennial ryegrass Tissue culture Plant regeneration |
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