In vitro study on the increase of trypsin resistance in the rat testicular sperm head. |
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| Authors: | T Matsuzawa T Hoshino J Ibuki |
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| Affiliation: | Laboratory of Biology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan. |
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| Abstract: | The alteration of trypsin resistance in rat sperm head was investigated after the sperm were incubated in the culture media (199-Earele, 1% BSA) containing the inhibitors of protein synthesis. The head of non-incubated testicular sperm was easily digested by trypsin (1 mg/ml) while the head of the incubated sperm showed resistance to trypsin digestion after 3 hr-incubation. Trypsin resistance in epididymal sperm head did not change after the sperm was incubated. Neither cycloheximide (100 micrograms/ml) nor chloramphenicol (100 micrograms/ml) affected the trypsin resistance in the testicular sperm head. |
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