| 两相分配法纯化受旱小麦绿色组织质膜的研究 |
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| 引用本文： | 李黎明,王韶堂.两相分配法纯化受旱小麦绿色组织质膜的研究[J].西北农林科技大学学报(社会科学版),1992,20(1):35-40. |
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| 作者姓名： | 李黎明 王韶堂 |
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| 作者单位： | 西北农业大学基础部，西北农业大学基础部 陕西杨陵·712100 陕西省烟草公司，陕西杨陵·712100 |
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| 摘 要： | 通过研究氯化钠和多聚物浓度对质膜和各种内膜在PEG/Dextran两相系统中分配行为的影响,根据其影响膜在两相系统中分配系数的试验结果,应用两相分配纯化法,纯化生长正常和受旱条件下小麦绿色组织质膜,对匀浆介质稍加修改,加入5mmol/L ZnCl_2,结果在较短的时间得到了两种小麦绿色组织细胞质膜的富集组分。
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| 关 键 词： | 两相分配纯化法 质膜纯化 内膜 水分胁迫 |
Studies on Purification of Plasma Membrane from Green Leaves of Wheat Under Water Stress by Two-Phase Partition |
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| Li Liming Wang Shaotang.Studies on Purification of Plasma Membrane from Green Leaves of Wheat Under Water Stress by Two-Phase Partition[J].Journal of Northwest Sci-Tech Univ of Agr and,1992,20(1):35-40. |
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| Authors: | Li Liming [Wang Shaotang ] |
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| Abstract: | The effects of sodium and polymer concentration on the partition behavious of plasma membrane and various inner-membrane in two-phase system of PEG / extran were studied. Based on the experimental results of its distribution coefficient in two-phase system of affected membrane, the purification method of two-phase system was used to purify the green plasma membrane of wheat growing in noormal and arid conditions. 5 mmol / L Nacl was added to modify the plasma medium. As a result, rich components of cell membrance of green tissues of wheat were obtained within the shortest possible time. |
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| Keywords: | two-phase partition purification method plasma membrane inner-membrane water stress |
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