| 小麦抗病种质贵农775中抗白粉病基因的RAPD标记 |
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| 引用本文： | 徐如宏,任明见,黄世全,杨英仓,张庆勤. 小麦抗病种质贵农775中抗白粉病基因的RAPD标记[J]. 作物学报, 2005, 31(2): 243-247 |
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| 作者姓名： | 徐如宏 任明见 黄世全 杨英仓 张庆勤 |
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| 作者单位： | 贵州大学麦作研究中心，贵州贵阳 550025 |
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| 基金项目： | 国家自然科学基金，贵州省小麦育种攻关项目，贵州省自然科学基金 |
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| 摘 要： | 运用RAPD技术，采用分离群体分组分析法（BSA）进行了小麦种质贵农775抗白粉病基因连锁的分子标记研究，其中有一个引物S2018在抗病亲本贵农775和抗病材料中扩增出了特异的DNA片段，而在感病材料和感病亲本丰产3号中没有扩增出同样的DNA片段。此片段长度约为880 bp。用F2分离群体（106株植株）进行遗传连锁性分析，引物S20188
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| 关 键 词： | 小麦 白粉病 抗性基因 分子标记 RAPD |
| 收稿时间： | 2003-10-24 |
| 修稿时间： | 2003-10-24 |
RAPD Markers Linked to the Powdery Mildew Resistance Gene in Wheat Line Gui-nong 775 |
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| XU Ru-hong,REN Ming-jian,HUANG Shi-quan,YANG Ying-cang,ZHANG Qing-qin. RAPD Markers Linked to the Powdery Mildew Resistance Gene in Wheat Line Gui-nong 775[J]. Acta Agronomica Sinica, 2005, 31(2): 243-247 |
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| Authors: | XU Ru-hong REN Ming-jian HUANG Shi-quan YANG Ying-cang ZHANG Qing-qin |
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| Affiliation: | Wheat research center of Guizhou University，Guiyang 550025，Guizhou |
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| Abstract: | Random Amplified Polymorphic DNA (RAPD) was employed to detect molecular markers linked to powdery mildew resistance gene in wheat line Gui-nong 775. One DNA fragment was amplified in the resistant parent Gui-nong 775 and resistant materials by a primer S2018, but not in the susceptible materials and susceptible parent Feng-chan 3. The DNA fragment length was about 880 bp. The genetic linkage of the DNA fragment with powdery mildew resistance gene was tested on a segregating F 2 population (106 plants). The result showed that the DNA fragment was closely linked to the powdery mildew resistance gene in wheat line Gui-nong 775, in which the genetic distance between S2018 880. and powdery mildew resistance gene was 8.9 cM. The linkage molecular markers were approved to be credible by other relative progeny materials. |
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| Keywords: | RAPD |
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