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| 亚麻不同阶段愈伤组织的蛋白组分初探 | |
| 引用本文： | 王克臣,冷超,黄文功,李冬梅,高波,李明.亚麻不同阶段愈伤组织的蛋白组分初探[J].东北农业大学学报,2008,39(11). |
| 作者姓名： | 王克臣 冷超 黄文功 李冬梅 高波 李明 |
| 作者单位： | 1. 东北农业大学农学院,哈尔滨,150030;哈尔滨学院,哈尔滨,150086 2. 黑龙江省农科院经作所,哈尔滨,150086 3. 东北农业大学农学院,哈尔滨,150030 |
| 摘 要： | 以黑亚14号和Opaline亚麻为试验材料,选取无菌苗、初始愈伤组织、胚性愈伤组织、非胚性愈伤组织、褐化组织5个阶段提取可溶性蛋白,通过对5个时期蛋白质进行SDS-PAGE分析,发现黑亚14号胚性愈伤组织在47ku处有一条特异性条带,Opaline胚性愈伤组织在50ku处有一条特异性条带,这两条特异性条带有可能作为各自的生化标记。 |
| 关 键 词： | 亚麻 愈伤组织 蛋白 |
Preliminary analysis on protein components of different callus in flax |
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| WANG Kechen,LENG Chao,HUANG Wengong,LI Dongmei,GAO Bo,LI Ming.Preliminary analysis on protein components of different callus in flax[J].Journal of Northeast Agricultural University,2008,39(11). | |
| Authors: | WANG Kechen LENG Chao HUANG Wengong LI Dongmei GAO Bo LI Ming |
| Institution: | WANG Kechen1,2,LENG Chao1,HUANG Wengong3,LI Dongmei1,GAO Bo1,LI Ming1 (1. College of Agriculture,Northeast Agricultural University,Harbin 150030,China,2. Academy of Harbin,Harbin 150086,3. Institute of Industrial Crops,HAAS,China) |
| Abstract: | Five different callus (aseptic plantlet, embryogenic callus, nonembryogenic callus, primary callus and browning tissue) of two varieties (Heiya 14 and Opaline) were selected to extract the soluble protein. Through assaying SDS-PAGE of 5 stages proteins, it showed that there was a specific peptide at 47 ku from Heiya 14 embryogenic callus. Furthermore, a specific peptide at 50 ku from Opaline embryogenic callus was observed. These two specific peptides may be used as their respective biochemical markers. |
| Keywords: | flax callus protein |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |