湖南省草莓灰霉病菌对4种杀菌剂的抗药性检测

为明确湖南省草莓灰霉病菌菌株的抗药性状况,2013-2015年从湖南省不同地区草莓灰霉病病果或病叶上经单孢分离获得草莓灰霉病菌454株,采用最小抑制浓度法(MIC)检测不同地区草莓灰霉病菌株对多菌灵、腐霉利、异菌脲、嘧霉胺的抗药性。结果表明:草莓灰霉病菌对多菌灵、腐霉利、异菌脲、嘧霉胺的抗性频率分别为55.73%,77.31%、3.52%和77.31%;所测菌株对4种杀菌剂的敏感性类型有Cbz~RPcm~RIpd~RPmt~R、CbzSPcm~RIpd~RPmt~R、Cbz~RPcm~RIpdSPmt~R、Cbz~SPcm~RIpdSPmt~S、Cbz~RPcm~SIpd~SPmt~R、Cbz~SPcm~RIpd~SPmt ~R等6种,其所占比例分别为33.26%、5.07%、41.41%、4.63%、3.96%、11.67%,未发现对4种杀菌剂均敏感的类型,表明湖南省草莓灰霉病菌已对多菌灵、腐霉利和嘧霉胺产生抗药性,对异菌脲的抗药性较低。

Detection of the resistance of Botrytis cinerea on strawberry in Hunan Province to four fungicides

To investigate the resistance of Botrytis cinerea to carbendazim, procymidone, iprodione and pyrimethanil, 454 strains of B.cinerea were collected from the diseased strawberry fruits or leaves in different regions of Hunan Province in 2013-2015 and resistance to the four fungicides were detected by minimal inhibitory concentration (MIC) method. The results showed that the resistance frequency of carbendazim, procymidone iprodione and pyrimethanil was 55.73%,77.31%, 3.52% and 77.31%, respectively. The susceptibility types were CbzRPcmRIpdRPmtR, CbzSPcmRIpdRPmtR, CbzRPcmRIpdSPmtR, CbzSPcmRIpdSPmtS, CbzRPcmSIpdSPmtR, CbzSPcmRIpdSPmtR, accounting for 33.26%, 5.07%, 41.41%, 4.63%, 3.96% and 11.67%, respectively. There was no type to be found that showed susceptible to all four fungicides. The results suggest that B.cinerea on strawberry plants in Hunan has produced resistance to carbendazim, procymidone and pyrimethanil,but susceptible to iprodione. Therefore, it is urgent to apply new fungicide or alternate fungicide with different mechanism to control B.cinerea.