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| 脉冲磁场对松材线虫生理生化及超微结构的影响 | |
| 引用本文： | 张明,宋晓斌,张星耀,王振朝,梁军. 脉冲磁场对松材线虫生理生化及超微结构的影响[J]. 林业科学, 2012, 48(3): 136-142 |
| 作者姓名： | 张明 宋晓斌 张星耀 王振朝 梁军 |
| 作者单位： | 1. 中国林业科学研究院森林生态环境与保护研究所国家林业局森林保护学重点实验室 北京100091;西北农林科技大学林学院 杨凌712100 2. 西北农林科技大学林学院 杨凌712100 3. 中国林业科学研究院森林生态环境与保护研究所国家林业局森林保护学重点实验室 北京100091 4. 河北大学电信学院 保定071002 |
| 摘 要： | 松材线虫病又称松树枯萎病,是由病原松材线虫(Bursaphelenchus xylophilus)引起的一种毁灭性森林病害。随着国际贸易日益频繁,松材线虫随木包装以及木材的进出口,反复传播的可能性越来越大(张星耀等,2003),而传统的熏蒸、热处理和杀线虫剂处理效率低、易造成环境污染(陈劲松等, |
| 关 键 词： | 脉冲磁场 脉冲前沿宽度 纤维素酶活性 死亡率 松材线虫 |
Effects of Pulsed Magnetic Field on the Biochemical and Physiological Characters and Ultrastructure of Pine Wood Nematode |
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| Zhang Ming , Song Xiaobin , Zhang Xingyao , Wang Zhenchao , Liang Jun. Effects of Pulsed Magnetic Field on the Biochemical and Physiological Characters and Ultrastructure of Pine Wood Nematode[J]. Scientia Silvae Sinicae, 2012, 48(3): 136-142 | |
| Authors: | Zhang Ming Song Xiaobin Zhang Xingyao Wang Zhenchao Liang Jun |
| Affiliation: | 1(1.Key Laboratory of Forest Protection of State Forestry Administration Research Institute of Forest Ecology,Environment and Protection,CAF Beijing 100091;2.College of Forestry,Northwest A&F University Yangling 712100;3.College of Electronic and Information Engineering,Hebei University Baoding 071002) |
| Abstract: | In this paper,we employed the pulsed magnetic field(PMF) processing to investigate the effect of different pulse rise time and treatment time on the survival,reproduction,cellulase activity and surface characteristics of the pine wood nematode(PWN).The results showed that the wider pulse and the longer treatment time were able to reduce the survival,and inhibit the cellulase activity and reproduction of PWN.The 4th instar and females had a strong resistance to the pulsed magnetic field.With pulse rise time of 10 ms and the treatment of 60 min,the adjusted mortality of PWN was 100%.After the PMF treatment,the cellulase activity of PWN increased within a short time,and then declined with the treatment time.The observation with a scanning electron micrograph(SEM) revealed that there was no significant change in morphology of PWN,but the quantity of rod-shaped bacteria carried by PWN was reduced. |
| Keywords: | pulsed magnetic field pulse rise time cellulase activity mortality rate pine wood nematode |
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