| 卷荚相思组培工厂化育苗技术 |
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| 引用本文： | 张月娇.卷荚相思组培工厂化育苗技术[J].林业科技开发,2012,26(1):100-103. |
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| 作者姓名： | 张月娇 |
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| 作者单位： | 福建省林业科技试验中心,福建南靖,363600 |
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| 基金项目： | 福建省林业厅种业创新与产业化工程项目 |
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| 摘 要： | 通过对卷荚相思组培工厂化育苗技术研究的阐述,总结了卷荚相思组培工厂化育苗最优培养基配方为:诱导培养基为改良MS+6-BA 0.3 mg/L+KT 0.5 mg/L+NAA 0.3 mg/L+蔗糖30 g/L+卡拉胶8 g/L,继代增殖培养基为改良MS+6-BA 0.8 mg/L+KT 0.3 mg/L+NAA 0.6 mg/L+蔗糖30 g/L+卡拉胶8 g/L,生根培养基为1/2改良MS+NAA 0.5 mg/L+蔗糖20 g/L+卡拉胶8 g/L,pH值为5.8。用此培养基配方进行卷荚相思组培工厂化育苗其诱导率可达70%,有效芽增殖倍数可达3倍以上,生根率可达95%以上。
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| 关 键 词： | 卷荚相思 组织培养 工厂化育苗 |
An industrialized technique for raising tissue culture plant of Acacia cincinnata |
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| ZHANG Yue-jiao.An industrialized technique for raising tissue culture plant of Acacia cincinnata[J].China Forestry Science and Technology,2012,26(1):100-103. |
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| Authors: | ZHANG Yue-jiao |
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| Institution: | ZHANG Yue-jiao Test Center of Fujian Forest Science & Technology,Nanjing 363600,Fujian,China |
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| Abstract: | A research on tissue culture technique of Acacia cincinnata was summarized and the optimal mediums were proposed in this paper.The medium of improved MS+6-BA 0.3mg/L+KT 0.5mg/L+NAA 0.3mg/L+sucrose 30 g/L+carrageenan 8g/L was optimal for inducement,the medium of improved MS+6-BA 0.8mg/L+KT 0.3mg/L+NAA 0.6mg/L+ sucrose 30 g/L+carrageenan 8 g/L was optimal for subculture.The medium of 1/2 improved MS+NAA 0.5mg/L+sucrose 20 g/L+carrageenan 8 g/L was optimal for rooting,and the pH was 5.8.Using these mediums,the inducement rate of industrialized tissue culture of Acacia cincinnata could be up to 70%,effective bud multiplication was more than three times,the rooting rate was more than 95%. |
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| Keywords: | Acacia cincinnata tissue culture industrialization for raising tissue culture plant |
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