转玉米PEPC基因杨树的光合生理特性分析

以转PEPC基因南林895杨和对照(南林895杨)为材料,分析不同转基因植株的光合生理参数。结果显示:转PEPC基因杨树与C4光合途径相关的酶活性比对照增高,PEPC活性增加较为明显,最高达38.6%。转PEPC基因杨树表现较强的对强光利用能力,其光饱和点比对照提高约10%～20%,饱和点时的光合速率比对照高17.7%～58.1%。转PEPC基因杨树光补偿点低于对照,利用弱光的能力增强;CO2饱和点低,比对照低22.2%～44.4%;CO2补偿点也较低,比对照降低58.1%～76.5%,表明对CO2的利用效率较高。此外,转基因杨树羧化效率增高,比对照增加最高达62.3%。

Analysis of Photosynthetic Characteristics of Transgenic Poplars with Maize PEPC Gene

In this study,transgenic poplars and the control(Populus deltoides × P.euramericana ’Nanlin895’) were used as testing materials,and physiological parameters of photosynthesis were analyzed.The results showed that the enzymes related to C4 photosynthesis in all the transgenic poplars increased compared with the control,and PEPC activity increased significantly as high as 38.6%.Also,transgenic poplars had strong capacity for using high light intensity,and its light saturation point increased by about 10% to 20%.The photosynthetic rates of transgenic poplars in light saturation point were higher than that of the control by a range of 17.74% to 58.1% and the light compensation points of transgenic poplars were lower than that of the control.Moreover,the ability of transgenic poplars to use low light was stronger than that of the control and CO2 saturation point of transgenic poplars was lower than the control by 22.2% to 44.4%.Transgenic poplars had lower CO2 compensation points than that of control by 58.1% to 76.5%,indicating that they had the ability for high-utilization of CO2.In addition,the transgenic poplars had higher efficiency of carboxylation,and the efficiency was up to 62.3% higher than that of the control.This study provided an experimental basis for high photosynthetic efficiency molecular breeding of poplars in the future.