松材线虫的低温冷冻保存

研究-80℃下松材线虫的冷冻保存方法,比较不同冷冻保护剂对松材线虫低温保存的保护效果,以及解冻后松材线虫的繁殖情况。结果表明:15%甘油对松材线虫保护效果最好,解冻后存活率达36.5%,其次为10%甘油、20%甘油、25%甘油,保护效果最差的是1%二甲基亚砜(DMSO);解冻后线虫能够正常生长繁殖,冻存3天和冻存30天的线虫存活率和繁殖无显著性差异。

Cryopreservation of Pinewood Nematode (Bursaphelenchus xylophilus)

In this study, we tested the cryopreservation of Bursaphelenchus xylophilus at-80 ℃, and investigated effects of different cryoprotectants on the survival and propagation. Cryopreservation with 15%(V/V) glycerol was found as the best protectant for cryopreservation, followed by 10%(V/V), 20%(V/V) and 25%(V/V)glycerol. The poorest protecting effect was with 1% dimethylsulfoxide. With 15%(V/V) glycerol, the survival rate reached to 36.5% after defrosting, and the nematodes retained normal propagation. There was no significant difference in the survival and propagation after cryopreservation between 3-days and 30-days cryopreservation which indicated that cryopreservation with 15%(V/V) glycerol was suitable for B. xylophilus at-80℃.