不同梓属树种花器官蛋白电泳分析

为了分析与楸树自交不亲和有关的特异蛋白质特性,采用考马斯亮蓝染色法和SDS-PAGE技术研究了2种梓属树种(滇楸、楸树)花器官的蛋白质含量及蛋白质组分。结果表明:楸树-1(连云港种源)的花柱、子房、花萼蛋白质含量较低,滇楸和楸树-2(老山种源)较高,而楸树-1花瓣蛋白质含量略高于其他树种;同一树种不同器官蛋白质含量表现为子房>花萼>花柱>花瓣。蛋白组分分析表明,梓属各树种不同花器官内的蛋白质主要集中在12～45 ku之间,各器官共有蛋白20个左右,发现具有特异性的蛋白包括:楸树-1花柱42 ku蛋白,楸树-2花柱64和19 ku蛋白,滇楸花柱37 ku蛋白,以及楸树-2和滇楸花柱共有的58和24 ku蛋白;楸树-1子房41、38和23 ku蛋白;花萼中,88和65 ku蛋白为楸树-1所有,52、40、26 ku蛋白为楸树-2和滇楸特有。花瓣内蛋白带较少,仅发现63 ku蛋白为楸树-1特异蛋白。

Analysis of protein in the different floral organs of Catalp genus by SDS-PAGE

To understand the mechanisms of self-incompatibility of Catalpa genus and its relationship with the specific proteins,the protein fractions and soluble proteins contents in the different floral organs of the three tree species of Catalp genus including C.fargesii,CB-1(C.bugeicorne come from Lianyun),CB-2(C.bugeicorne come from Laoshan)] were studied by the techniques of SDS-PAGE and Brilliant Blue and Commassie Dye.The results showed that the soluble proteins contents in the style,ovary and calyx of CB-1 were lower than that of C.fargesii and CB-2,while the soluble proteins contents in the petal of CB-1 were higher than that of the other species.For each of three species,the soluble proteins contents in the organs were in the order at ovary>calyx>style>petal.There were 20 proteins bands observed in the floral organs of three species and the MW(relative molecular weight) were mainly from 12 to 45 ku.By the techniques of SDS-PAGE,some specific proteins were found in the different floral organs of the species.In the style,the 42 ku protein was found in CB-1,the 64 and 19 ku proteins in CB-2,and the 37 ku protein in C.fargesii.The 58 and 24 ku proteins were found both in CB-2 and in C.fargesii.The 41,38 and 23 ku proteins were found in the ovary of CB-1.In the calyx,the 88 and 65 ku proteins were only found in CB-1,and the 52,40 and 26 ku proteins was found in CB-2 and C.fargesii.While in the petal,only 63 ku protein was found in CB-1.