桑天牛幼虫感染球孢白僵菌后蛋白质和氨基酸含量的变化

桑天牛现称桑粒肩天牛(Apriona germari),是多种林木、果树、花卉的重要蛀干害虫,也是我国危害最严重、最难防治的害虫之一。近年来,其猖獗危害已成为我国养蚕业、林业及果树种植业等经济发展的严重障碍。球孢白僵菌(Beauveria bassiana)是一类广谱性昆虫病原真菌,能侵染15目149科的700

Changes in the Contents of Proteins and Amino Acids of Apriona germari Larvae Infected by Beauveria bassiana

To understand the relationship between the contents of proteins and free amino acids and the infection of Beauveria bassiana.Changes in the contents of proteins and free amino acids in haemolymph of Apriona germari larvae infected by B.bassiana were detected.The result showed that the content of proteins increased to the maximum in 3 days after inoculation,indicating the proteins and enzymes could be produced for the immunologic responses,and then declined.By using the polyacrylamide gelelectrophoresis(PAGE)technique,the change in protein composition in Apriona germari larvae infected by Beauveria bassiana was analyzed.The result showed that the band number increased after inoculation.There were significant differences in the band widths and staining intensities.The results of amino acids analysis showed that there were abundant necessary amino acids for the strain’s growth in A. germari larvae bodies,thus B. bassiana showed a high virulence to A. germari larvae.The content of free amino acids decreased in 24 h after inoculation,suggesting that the B. bassiana invaded into the insect body absorbed some amino acids from the blood as nutrition.The content of free amino acids had no further changes from 24-72 h,and then increased sharply in 96 h and 120 h probably because of gradual loss ofimmunoregulation,destroy of tissue and disintegration of proteins.Before dead of the infected A. germari larvae,the contents of different amino acids changed differentially,suggesting that different growth phases of the isolate needed different amino acids.