| 茛艻花组织培养技术 |
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| 引用本文： | 吕秀立.茛艻花组织培养技术[J].林业科技开发,2012,26(3):114-117. |
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| 作者姓名： | 吕秀立 |
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| 作者单位： | 上海市园林科学研究所,上海,200232 |
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| 基金项目： | 上海市科技兴农重点攻关项目 |
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| 摘 要： | 以茛艻花顶芽为外植体进行离体培养,成功建立了快速繁殖技术体系,结果表明,不同激素及其浓度对其增殖及根的形成影响显著。各个阶段适宜培养基:(1)增殖培养基为MS+BA 3.0 mg/L+NAA 1.0 mg/L,培养30天,增殖率稳定为4.65;(2)壮苗培养基为MS+6-BA 0.2 mg/L+IBA 0.2 mg/L;(3)生根培养基为1/2MS+NAA4mg/L+蔗糖20 g/L时,生根率达95%。
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| 关 键 词： | 茛艻花 离体培养 快速繁殖 |
Tissue culture techniques for Acanthus mollis in vitro |
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| L Xiu-li.Tissue culture techniques for Acanthus mollis in vitro[J].China Forestry Science and Technology,2012,26(3):114-117. |
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| Authors: | L Xiu-li |
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| Institution: | L(ü) Xiu-li |
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| Abstract: | Tissue culture techniques of Acanthus mollis was developed by using shoot tips as explants.The results showed that the concentration of different plant growth regulators affected bud differentiation and rooting of plantlets.The optimum differentiation medium was MS+BA3.0 mg/L+NAA1.0 mg/L,the multiplication rate was 4.65.The optimum culture medium was MS+ 6-BA0.2 mg/L+IBA0.2mg/L for strong of the buds.For rooting,the optimum medium was 1/2MS+NAA4mg/L +sucrose20g/L,the rooting rate was 95%. |
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| Keywords: | Acanthus mollis tissue culture in vitro rapid propagation |
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