| 人参根际土壤微生物RAPD反应体系的建立 |
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| 引用本文： | 应益昕,丁万隆,李勇.人参根际土壤微生物RAPD反应体系的建立[J].中国农学通报,2010,26(7):36-39. |
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| 作者姓名： | 应益昕 丁万隆 李勇 |
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| 作者单位： | 中国医学科学院北京协和医学院药用植物研究所,北京,100193 |
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| 基金项目： | 高等学校博士学科点专项科研基金课题 |
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| 摘 要： | 本研究建立了一种适合于开展参地土壤微生物遗传多样性的RAPD反应体系。利用优化的RAPD反应体系，对200条RAPD引物进行了筛选，其中38条引物多态性高、扩增结果稳定。在此基础上，用筛选出的多态性RAPD引物对参地土壤微生物总DNA进行了扩增。试验结果表明，本研究建立的RAPD反应体系适合开展人参根际土壤微生物的遗传多样性研究。
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| 关 键 词： | 层次分析法 层次分析法 物流园区 |
| 收稿时间： | 2009-12-11 |
| 修稿时间： | 2009-12-18 |
Establishment of RAPD Reaction System for Panax ginseng Rhizosphere Soil Microbial Community |
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| Ying Yixin,Ding Wanlong,Li Yong.Establishment of RAPD Reaction System for Panax ginseng Rhizosphere Soil Microbial Community[J].Chinese Agricultural Science Bulletin,2010,26(7):36-39. |
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| Authors: | Ying Yixin Ding Wanlong Li Yong |
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| Institution: | Ying Yixin,Ding Wanlong,Li Yong (Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences , Peking Union Medical College,Beijing 100193) |
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| Abstract: | To establish RAPD reaction system that fitted for genetic diversity study of Panax ginseng rhizosphere soil microbial community, and screen polymorphic RAPD primers by optimized RAPD reaction system;. According to reports published, optimize soil microbial RAPD reaction system and amplification program, and fitted for genetic diversity study ofP. ginseng rhizosphere soil microbial community. By optimized universal RAPD reaction system, 38 out of 200 RAPD primers having high polymorphism and amplified stably... |
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| Keywords: | RAPD |
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