东北对开蕨两种繁殖技术的研究

以东北对开蕨（Phyllitis japonica Kom．）的孢子叶为繁殖材料，进行组织培养繁殖和常规人工播种繁殖。结果表明：不同的繁殖方法对东北对开蕨孢子的萌发和原叶体的增殖有明显影响，组织培养繁殖的孢子成活率较低但原叶体增殖率为45％；人工播种繁殖的孢子萌发情况很好但原叶体的增殖率为34％。从东北对开蕨完整植株形成来看，组织培养技术更适宜其生长。

The Study on Two Reproduction Techniques of Phyllitis japonica

The situations of micropropagation in tissue culture and artificial reproduction of Phyllitis japonica Kom. with the explant of sporophyl are researched. The experimental results indicated that different reproduction methods had obvious effects on spore germination and prothallus proliferation of Phyllitis japonica Kom. The surivival rate with tissue culture reproduction is low, but its proliferation rate is 45%;the spore germination condition with artificial sowing reproduction is well, its proliferation rate of spore is 34%.Seen from the whole plant formation of Phyllitis japonica Kom, the tissue culture tenhnique is more suitable for its growth.