Molecular cloning of cDNA of mammalian and chicken II gonadotropin-releasing hormones (mGnRHs and cGnRH-II) in the beluga (<Emphasis Type="Italic">Huso huso</Emphasis>) and the disruptive effect of methylmercury on gene expression |
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Authors: | Ahmad Gharaei Fereidoun Mahboudi Abbas Esmaili-Sari Rozita Edalat Ahmad Adeli Saeed Keyvanshokooh |
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Institution: | (1) Department of Fisheries, Faculty of Natural Resources, Universityof Zabol, P.O.Box 98615-538, Zabol, Sistan & Blouchestan, Iran;(2) Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran;(3) Department of Environment, Faculty of Natural Resources and Marine Science, Tarbiat Modares University, Noor, Mazandaran, Iran;(4) Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Khouzestan, Iran |
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Abstract: | Two gonadotropin-releasing hormone (GnRH) isoforms were identified in the beluga (Huso huso) brain by cDNA sequencing: prepro-mammalian GnRH (mGnRH) and prepro-chicken GnRH-II (cGnRH-II). The nucleotide sequences
of the beluga mGnRH and cGnRH-II precursors are 273 and 258 base pairs (bp) long, encoding peptides of 91 and 86 amino acids,
respectively. To investigate the effect of methylmercury (MeHg) on GnRH gene expression, animals were fed with four diets
containing increasing levels of MeHg (0 mg kg−1 control]; 0.76 mg kg−1 low]; 7.8 mg kg−1 medium]; 16.22 mg kg−1 high]) for 32 days. The effects of MeHg on brain GnRH mRNA levels were evaluated by real-time PCR. A significant decrease
in brain mGnRH and cGnRH-II mRNA levels were detected in fish receiving high dietary MeHg dose compared to controls on day
11 (P < 0.05). On day 18 and 32, all treatment groups had significantly lower brain mGnRH and cGnRH-II mRNA levels compared to
the control group (P < 0.05). These findings demonstrate a disruptive role of MeHg on the level of brain mGnRH and cGnRH-II mRNAs in immature
beluga. |
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