| 甜樱桃MADS box基因的克隆与表达分析 |
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| 引用本文： | 林苗苗,赵长竹,姜建福,顾红,陈锦永,方金豹.甜樱桃MADS box基因的克隆与表达分析[J].园艺学报,2011,38(8):1462-1466. |
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| 作者姓名： | 林苗苗 赵长竹 姜建福 顾红 陈锦永 方金豹 |
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| 作者单位： | 中国农业科学院郑州果树研究所，中国农业科学院果树生长发育与品质控制重点开放实验室，郑州 450009 |
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| 基金项目： | ‘十一五’国家科技支撑计划项目(2006BAD07B06-10); 中国农业科学院果树生长发育与品质控制重点开放实验室项目 |
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| 摘 要： | 以甜樱桃‘拉宾斯’（Prunus avium L.‘Lapins’）为试材，采用RT-PCR结合RACE技术，克隆获得1个与花发育相关的MADS box基因，命名为PaMADS3，其在GenBank中的登录号为HQ229605。PaMADS3基因全长1 095 bp，包含1个723 bp的开放阅读框，推断其编码240个氨基酸。序列分析表明：PaMADS3蛋白与拟南芥中的SEP蛋白高度同源。组织特异性表达显示PaMADS3基因在花瓣、雄蕊、心皮中表达。实时定量RT-PCR分析表明，花芽露绿期的离体枝条经过15和25 ℃处理后，其雌蕊中PaMADS3基因在25 ℃的表达量高于在15 ℃的表达量。
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| 关 键 词： | 甜樱桃 花发育 MADS box 克隆 表达 |
| 收稿时间： | 2011-5-17 |
| 修稿时间： | 2011-7-19 |
Cloning and Expression Analysis of MADS box Gene in Sweet Cherry(Prunus avium) |
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| LIN Miao-miao,ZHAO Chang-zhu,JIANG Jian-fu,GU Hong,CHEN Jin-yong, FANG Jin-bao.Cloning and Expression Analysis of MADS box Gene in Sweet Cherry(Prunus avium)[J].Acta Horticulturae Sinica,2011,38(8):1462-1466. |
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| Authors: | LIN Miao-miao ZHAO Chang-zhu JIANG Jian-fu GU Hong CHEN Jin-yong FANG Jin-bao |
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| Institution: | 1. Faculty of Dentistry, School of Medicine, Jinan University, Guangzhou 510632, China;
2. Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China;
3. Department of Stomatology, Shenzhen Children's Hospital, Shenzhen 518026, China |
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| Abstract: | A MADS box gene was isolated from the floral bud of sweet cherry(Prunus avium L.'Lapins')by using RT-PCR and RACE approaches,the gene was named PaMADS3(The accession number in GenBank:HQ229605).The full length cDNA of PaMADS3 is 1 095 bp,with an ORF of 723 bp encoding 240 putative amino acid residues.Sequence analysis indicated that PaMADS3 shared highly homology with Arabidopsis SEP gene.Tissue specific analysis showed that PaMADS3 expressed in petal,stamen,and carpel.Real-time RT-PCR revealed that when th... |
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| Keywords: | sweet cherry flower development MADS box cloning expression |
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