| 水稻耐盐性SSR标记引物的筛选 |
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| 引用本文： | 张燕红,王奉斌,袁杰,布哈丽且木·阿布力孜,文孝荣,梁乃亭.水稻耐盐性SSR标记引物的筛选[J].新疆农业科学,2011,48(6):1064-1069. |
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| 作者姓名： | 张燕红 王奉斌 袁杰 布哈丽且木·阿布力孜 文孝荣 梁乃亭 |
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| 作者单位： | 新疆农业科学院核技术生物技术研究所,乌鲁木齐,830091;新疆农业科学院温宿水稻试验站,新疆,温宿,843000 |
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| 基金项目： | 新疆维吾尔自治区高技术研究发展计划项目，新疆维吾尔自治区科技成果转化专项基金项目 |
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| 摘 要： | 目的]针对两个典型的水稻品种(系)耐盐材料(02-11和津源85)和两个敏感材料(秋田小町和99-28),进行水稻耐盐相关的SSR标记的研究.方法]选取水稻12条染色体上的565对SSR引物进行亲本间多态性筛选;选择适合的标记引物,并确定PCR扩增的最适退火温度.结果]在565对引物组合中筛选出68对引物在4个材料间有多态性;明确了主要标记引物的通用退火温度为55℃.结论]68对可作为该水稻群体耐盐QTL分析的SSR标记引物,为进一步进行水稻耐盐性分子鉴定和耐盐分子辅助育种提供参考.
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| 关 键 词： | 水稻 耐盐性 SSR 引物筛选 |
| 收稿时间： | 2011-06-25 |
Screening of Salinity-tolerance with SSR Markers on Rice |
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| MANG Yan-hong,WANG Feng-bin,YUAN Jie,Buhaliqiemu Abulizi,WEN Xiao-rong,LIANG Nai-ting.Screening of Salinity-tolerance with SSR Markers on Rice[J].Xinjiang Agricultural Sciences,2011,48(6):1064-1069. |
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| Authors: | MANG Yan-hong WANG Feng-bin YUAN Jie Buhaliqiemu Abulizi WEN Xiao-rong LIANG Nai-ting |
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| Institution: | ZHANG Yan-hong1,WANG Feng-bin1,YUAN Jie1,Buhaliqiemu Abulizi1,WEN Xiao-rong2,LIANG Nai-ting1(1.Research Institute of Nuclear and Biological Technologies,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China,2.Wensu Rice Experiment Station,Wunsu Xinjiang 843000,China) |
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| Abstract: | 【Objective】 The purpose of this study is to analyze the salinity-related SSR markers by applying SSR method to test the two typical salinity tolerance varieties(02-11 and Jinyuan 85) and the two typical salinity sensitive varieties(Qiutian Xiaoding and 99-28).【Method】A total of 565 polymorphic SSR markers on 12 chromosomes were applied to screen suitable primer on the four parents and the annealing temperature.【Result】Sixty-eight pairs of SSR polymorphism primers were obtained from 565 pairs of SSR primers.It was confirmed that the suitable annealing temperature was at 55℃.【Conclusion】Sixtyeight pairs of SSR marker were used to analyze salinity-tolerance of rice varieties.It laid the foundation for molecular identify and molecular marker-assisted breeding of salinity-tolerance of rice. |
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| Keywords: | rice salinity-tolerance SSR primer screening |
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