提高玉米秸秆纤维素酶解率的预处理研究

[目的]比较使用不同预处理方法对媒介纤维素水解率的影响.[方法]用稀酸法、稀碱法、亚钠法对玉米秸秆进行预处理,再用纤维素酶对玉米秸秆中纤维素进行水解.[结果]在50℃、pH为4.8、固液比为1:30、酶浓度为2.7g/L、反应时间为24h的条件下,可获得较理想的酶解率.经亚钠预处理后的玉米秸秆,纤维素含量上升最多,酶解率最高,亚钠预处理后的酶解率达到39.07;,是未经处理的秸秆酶解率(9.8;)的4倍.[结论]预处理破坏了玉米秸秆的纤维素结构.采用亚钠法-酶法结合工艺处理玉米秸秆进行纤维素酶解可显著提高酶解率.

Study on Pretreatment of Improving Corn Stalk Enzymatic Hydrolysis Yield

【Objective】Three different pretreatment methods were tested to study the effect on hydrolysis of cellulose from corn stalk.【Method】The dilute-acid,dilute-alkali and sodium sulfite anhydrous pretreatment of corn stalk were carried out,followed by cellulose enzymatic hydrolysis.【Result】The results showed that the optimum condition for enzymatic hydrolysis is at temperature of 50℃,pH of 4.8,ratio of solid to liquid 1∶30,enzyme concentration of 2.7 g/L,and the hydrolysis time of 24 h.Under the optimized condition,the rate of enzymatic hydrolysis on corn stalk pretreatment using sodium sulfite anhydrous was 39.07%,which is four multiple higher than that of corn stalk hydrolysis without pretreatment.【Conclusion】Pretreatment destroyed the fibrous structure of corn stalk significantly.Relative good rate of enzymatic hydrolysis on corn stalk can be achieved by sodium sulfite anhydrous pretreatment.