| 十字花科植物HRD基因的克隆及分析 |
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| 引用本文： | 刘瑞娜,焦天奇,王爱英,祝建波,张煜星.十字花科植物HRD基因的克隆及分析[J].新疆农业科学,2011,48(3):437-444. |
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| 作者姓名： | 刘瑞娜 焦天奇 王爱英 祝建波 张煜星 |
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| 作者单位： | 石河子大学生命科学学院,农业生物技术重点实验室,新疆石河子,832003 |
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| 基金项目： | 国家转基因专项，新疆生产建设兵团博士基金 |
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| 摘 要： | 目的]探索克隆出的十字花科植物基因同拟南芥HRD基因之间是否具有同源性,是否为十字花科植物的家族基因.方法]利用分子生物学的方法从十字花科植株中克隆出核酸序列,并对克隆出的序列进行分析.结果]十字花科植株中克隆出的基因同拟南芥的HRD基因的核酸序列和氨基酸序列具有很大的相似性,生物进化树分析表明它们具有同源性.结论]拟南芥的HRD基因和十字花科植物中克隆出的序列是由同一个基因进化而来,是十字花科植物的保守基因.
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| 关 键 词： | HRD 转录因子 分子生物学 进化 |
| 收稿时间： | 2011-03-25 |
HRD Gene Cloning and Analysis of Cruciferous |
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| LIU Rui-na,JIAO Tian-qi,WANG Ai-ying,ZHU Jian-bo,ZHANG Yu-xing.HRD Gene Cloning and Analysis of Cruciferous[J].Xinjiang Agricultural Sciences,2011,48(3):437-444. |
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| Authors: | LIU Rui-na JIAO Tian-qi WANG Ai-ying ZHU Jian-bo ZHANG Yu-xing |
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| Institution: | LIU Rui-na,JIAO Tian-qi,WANG Ai-ying,ZHU Jian-bo,ZHANG Yu-xing(Laboratory of Agricultural Biotechnology,College of Life Science,Shihhotze University,Shihhotze,Xinjiang 832003,China) |
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| Abstract: | 【Objective】The purpose of the research was to reveal the homology between the cloned gene of Cruciferous and Arabidopisis HRD gene to confirm whether HRD gene is Cruciferous's conservative gene or not.【Method】The nucleicacid sequence from the plants of Cruciferous was cloned and analysed using the method of molecular biology.【Result】The result shows that their DNA sequence and amino acid sequences has great similarities,and biological evolutionary tree analysis shows that they have the homology.【Conclusion】Arabidopisis HRD gene and the cloned sequence from the Cruciferous has the same ancestor,it is a conservative gene of Cruciferous. |
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