| 党参无菌苗培养前种子消毒技术研究 |
| |
| 引用本文： | 薛雯心,魏铁锁,袁金月,刘立业,姜雪琪,李秀霞. 党参无菌苗培养前种子消毒技术研究[J]. 安徽农业科学, 2017, 45(12). DOI: 10.3969/j.issn.0517-6611.2017.12.044 |
| |
| 作者姓名： | 薛雯心 魏铁锁 袁金月 刘立业 姜雪琪 李秀霞 |
| |
| 作者单位： | 佳木斯大学生命科学学院,黑龙江佳木斯,154007;佳木斯大学生命科学学院,黑龙江佳木斯,154007;佳木斯大学生命科学学院,黑龙江佳木斯,154007;佳木斯大学生命科学学院,黑龙江佳木斯,154007;佳木斯大学生命科学学院,黑龙江佳木斯,154007;佳木斯大学生命科学学院,黑龙江佳木斯,154007 |
| |
| 基金项目： | 国家级大学生创新创业训练计划项目 |
| |
| 摘 要： | [目的]研究不同消毒方法下党参种子的消毒效果。[方法]以党参种子为试验材料,通过10%H_2O_2、0.1%HgCl_2和2%NaClO这3种消毒剂,75%乙醇预处理,无菌水预浸泡,对党参种子的消毒效果进行比较。[结果]在10%H_2O_2、0.1%HgCl_2和2%NaClO这3种消毒剂中,2%NaClO浸种20 min的消毒效果最好;用75%乙醇预处理20 s可降低种子带菌率;在党参种子消毒之前,无菌水预浸泡24 h可提高发芽率。[结论]该研究结果为培育党参无菌苗等研究提供了重要依据。
|
| 关 键 词： | 党参 种子消毒 无菌苗 |
Methods for Seed Disinfection before Culturing Aseptic Codonopsis pilosula Seedling |
| |
| XUE Wen-xin,WEI Tie-suo,YUAN Jin-yue,LIU Li-ye,JIANG Xue-qi,LI Xiu-xia. Methods for Seed Disinfection before Culturing Aseptic Codonopsis pilosula Seedling[J]. Journal of Anhui Agricultural Sciences, 2017, 45(12). DOI: 10.3969/j.issn.0517-6611.2017.12.044 |
| |
| Authors: | XUE Wen-xin WEI Tie-suo YUAN Jin-yue LIU Li-ye JIANG Xue-qi LI Xiu-xia |
| |
| Abstract: | [Objective]To study the disinfection effects of Codonopsis pilosula seedling under different disinfection treatments.[Method]Taking C.pilosula seeds as test materials,through three kinds of disinfectants 10%H2O2, 0.1% HgCl2 and 2% NaClO,pretreatment with 75% ethanol and presoaking with sterile water,the effects of C.pilosula seed sterilization were studied.[Result]2%NaClO had the greatest sterilization effect.The germ-carring rate of seed was decreased with pretreatment of 75% ethanol.The seeds were soaked in aseptic water for 24 hours before sterilization,the seeds germination rate could increase.[Conclusion]The results of this study provide an important basis for the research on the cultivation of sterile seedlings of C.pilosula. |
| |
| Keywords: | Codonopsis pilosula Seed disinfection Aseptic seedling |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
