GnRHR在不同日龄建昌黑山羊睾丸、附睾中的分布与表达

目的]定位并比较不同日龄建昌黑山羊睾丸及附睾中GnRHR的分布及表达情况。方法]分别收集55、104、300、1 140日龄建昌黑山羊的睾丸、附睾头和附睾尾组织,然后进行脱水、包埋、切片后,采用免疫组化定位GnRHR在各个组织中的分布。结果]GnRHR在建昌黑山羊睾丸、附睾头和附睾尾组织细胞中均有分布,在上皮下层、基质层中分布较多,其表达量在300日龄的附睾尾和1 140日龄的睾丸中达到较高水平。结论]GnRHR在睾丸、附睾中均有表达,而且不同日龄阶段表达存在组织细胞特异性。

Localization and Distribution of GnRHR in the Testis and Epididymis from Different Age of Jianchang Black Goat

Objective] To localize and compare the distribution and expression level of GnRHR in the testis and epididymis from different age of Jianchang black goat.Method]The testis and epididymis were collected at 55,104,104,1 140 d after animal birth,then dehydrated,embedded and cut.Distribution of GnRHR was orientated by immunohistochemical assay.Result] GnRHR in the testis,epididymal heads and tails,especially in subepithelial layer and stroma layer,in which had a higher expression level than other tissue was expressed.GnRHR expression was the most in the epididymis at 300 d and in the testis at 1 140 d.Conclusion] GnRHR is expressed in the testis and epididymis,and its expression level has tissue-specificity at different age.