模拟不同因素对狼蛛嗅觉器官定位猎物的影响

目的]了解狼蛛捕食行为的影响因子。方法]以拟水狼蛛为研究对象,采用L_9(3~4)正交试验设计,探讨了狼蛛嗅觉感受器对捕食行为(寻觅、选择与定位猎物)的影响,并对不同反应观察时长提出试验设计方案。试验全过程在25℃恒温环境完成,采用四臂嗅觉仪测定。结果]间距对狼蛛定位果蝇灵敏度的影响最大,其次是果蝇组织液浓度和狼蛛反应时长观察。经正交试验统计检验,得出狼蛛定位猎物的最佳优化组合为果蝇体液浓度80%、距离为6 cm、狼蛛反应时长300 s,此时狼蛛嗅觉感受器灵敏度最高。结论]试验结果为制定保护和利用蜘蛛防治害虫的措施提供了理论依据。

Effects of Olfactory Receptor on Locating Prey of Pirata subpiraticus in Different Factors

Objective] The aim was to understand influencing factors of feeding behavior of Pirata subpiraticus.Method] We used L9(34) orthogonal experiment for Pirata subpiraticus's olfactory receptor and tested at 25 ℃ environment to explore factors that Pirata subpiraticus's olfactory receptors how to find,to select and locate prey(fruit fly) through several experiments,and used four-arm olfactometer experimental device to explore the accuracy in predation prey of the Pirata subpiraticus and proposed experimental design options for different experimental observations with Pirata subpiraticus's reaction.Result] The distance had the biggest effect on the chemoreceptor sensitivity of Pirata subpiraticus,secondly were fruit fly fluid concentration and experimental observation.The optimal combination that Pirata subpiraticus's olfactory receptors appeared the highest sensitivity was as followed: the fruit fly fluid concentration of 80%,a distance of 6 cm,the experimental observation of 300 s reaction time.Conclusion] The results provide theoretical basis for making measures for protecting and using Pirata subpiraticus to control pests.