石橄榄种子无菌播种和快速繁殖研究 |
| |
引用本文: | 林辉锋,陈昌铭,尚伟,林发壮,夏朝水,周辉明. 石橄榄种子无菌播种和快速繁殖研究[J]. 安徽农业科学, 2017, 45(8). DOI: 10.3969/j.issn.0517-6611.2017.08.054 |
| |
作者姓名: | 林辉锋 陈昌铭 尚伟 林发壮 夏朝水 周辉明 |
| |
作者单位: | 福建省三明市农业科学研究院,福建三明,365051;福建省三明市农业科学研究院,福建三明,365051;福建省三明市农业科学研究院,福建三明,365051;福建省三明市农业科学研究院,福建三明,365051;福建省三明市农业科学研究院,福建三明,365051;福建省三明市农业科学研究院,福建三明,365051 |
| |
摘 要: | [目的]建立石橄榄种子无菌播种和快速繁殖技术体系。[方法]以MS为基础培养基,添加不同浓度6-BA、NAA、有机添加物,筛选适宜的诱导、增殖及壮苗生根培养基组合。[结果]以MS+6-BA 1.0 mg/L+NAA 0.5 mg/L+椰子汁50 mL/L为石橄榄种子最佳诱导培养基,14 d可见种子萌动,45 d可分化形成丛生幼苗;以MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+椰子汁50 mL/L为最佳增殖培养基,其增殖倍数可达2.750倍,假鳞茎形成率达24.5%;以1/2MS+NAA 0.1 mg/L+AC 1 g/L为最佳壮苗生根培养基,苗高可达3.82 cm,根数1.9条。[结论]该研究为石橄榄野生种质资源保护、快速繁育优质种苗奠定了基础。
|
关 键 词: | 石橄榄 无菌播种 组织培养 |
Study on Aseptic Sowing and Rapid Propagation Technology for Pholidota chinensis Lindl.Seed |
| |
Abstract: | [Objective] To establish a system of aseptic sowing and rapid progagation technology for Pholidota chinensis Lindl..[Method]Different concentrations 6-BA, NAA, organic additives were added to MS medium to screen the suitable induction, proliferation and strong seedling and rooting medium.[Result]The proper medium for the seeds induction of P.chinensis Lindl.was MS medium containing 6-BA 1.0 mg/L+ NAA 0.5 mg/L + coconut juice 50 mL/L, seed germination was observed on 14 days,the differentiation and formation of clusters of seedlings were observed on 45 days;the best multiplication medium was MS medium containing 6-BA 1.0 mg/L+NAA 0.1 mg/L+coconut juice 50 mL/L,proliferation times 2.750,the pseudobulb formation rate up to 24.5%;the best seedling rooting medium was 1/2MS medium containing NAA 0.1 mg/L+AC 1 g/L,plant length up to 3.82 cm,root number 1.9.[Conclusion]The study laid a foundation for the conservation of wild germplasm resources and rapid breeding of high quality seedling. |
| |
Keywords: | Pholidota chinensis Lindl. Aseptic sowing Tissue culture |
本文献已被 CNKI 万方数据 等数据库收录! |
|