液相色谱-同位素质谱法鉴定蜂蜜掺假

目的]探讨液相色谱-同位素质谱法(LC-IRMS)技术在蜂蜜掺假鉴定中的应用效果。方法]采用LC-IRMS法检测随机抽样的62个蜂蜜样品,对蜂蜜中果糖(Fru)、葡萄糖(Glu)、二糖和三糖δ~(13)C值进行测定。结果]由于真实蜂蜜中果糖、葡萄糖、二糖和三糖的δ~(13)C的最大值与最小值的差值小于2.1‰,果糖、葡萄糖的δ~(13)C差值(δ~(13)CFru-δ~(13)CGlu)为-1‰~1‰,且不得含有寡糖,所以该次检测样品中有48.38%的蜂蜜不合格,掺假掺杂以添加C-3植物源转化产物和淀粉糖浆为主,应加强对这类掺假的监控管理。结论]LC-IRMS实现了蜂蜜中各种糖组分如果糖、葡萄糖、二糖和三糖等δ~(13)C的分离与分析,可进行添加了C-3糖浆的掺假鉴定,大大提高了蜂蜜掺假的检测能力。

Identification of Honey Adulteration by Liquid Chromatography-isotope Mass Spectrometry (LC-IRMS)

Objective] The aim was to explore application of LC-IRMS technique in identification of honey adulteration.Method] We detected 62 honey samples by LC-IRMS, and determined δ13C value of fructose, glucose, disaccharide and trisaccharide of honey.Result] The difference of maximum and minimum δ13C of fructose, glucose, disaccharide and trisaccharide of true honey was less than 2.1‰, and δ13CFru-δ13CGlu was from-1‰ to 1‰, and there was no oligosaccharides, so 48.38% of samples was no qualified.The adulteration was mainly C-3 plant products and starch syrup, so the monitoring management of adulteration should be strengthened.Conclusion] LC-IRMS realizes the δ13C isolation and analysis of fructose, glucose, disaccharide and trisaccharide of honey, so it can be used in the adulteration identification of C-3 syrup and can improve the detection capability of honey adulteration.