| 金针菇原生质体紫外诱变选育 |
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| 引用本文： | 成亚利,朱宝成.金针菇原生质体紫外诱变选育[J].食用菌学报,1995,2(3):61-64. |
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| 作者姓名： | 成亚利 朱宝成 |
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| 作者单位： | 河北大学生物系，河北大学生物系，河北大学生物系，河北大学生物系 保定 071002，保定 071002，保定 071002，保定 071002 |
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| 摘 要： | 本研究在最适条件下制备金针菇Ｙ１９和Ｗ０８８菌株原生质体，紫外线照射１分钟进行诱变处理，再生后挑选生长好的菌株，酯酶同工酶电泳分析表明，筛选出的菌株出现出发株没有的新酶带，证明其遗传物质发生了变化，与出发菌株相比，变异菌株生长速度提高２３．１￣５２．３％，出菇时间提早１０￣１４天。
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| 关 键 词： | 金针菇 原生质体 诱变 |
A Study on Ultraviolet Mutagenesis of Flammulina velutipes Protoplasts |
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| Cheng Yali Zhu Baocheng Li Liangliang Li Qingyu.A Study on Ultraviolet Mutagenesis of Flammulina velutipes Protoplasts[J].Acta Edulis Fungi,1995,2(3):61-64. |
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| Authors: | Cheng Yali Zhu Baocheng Li Liangliang Li Qingyu |
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| Abstract: | The protoplasts of Flammulina velutipes obtained under optimum conditions were treated with UV-irradiation for 1 min. After the regeneration of the protoplasts, strains with better growth rate were selected. And with the aid of esterase isozyme electrophoresis, new bands different from parent strains were found in selected strains. This indicated that the genetical materials of selected strains were different from those of their parent strains. Compared with the parent strains, the mycelia growth rate of mutagenic strains increased 23.1 %-52.3% and their fruiting period was 10-14 days earlier. |
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| Keywords: | Flammulina velutipes Protoplast Mutagenesis |
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