| 农杆菌介导的SeNHX1基因转化月季愈伤组织的研究 |
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| 引用本文： | 刘会超,刘孟刚,郭丽娟,贾文庆.农杆菌介导的SeNHX1基因转化月季愈伤组织的研究[J].林业科学研究,2010,23(4):617-621. |
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| 作者姓名： | 刘会超 刘孟刚 郭丽娟 贾文庆 |
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| 作者单位： | 1. 河南科技学院园林学院,河南,新乡,453003
2. 河南科技学院新科学院,河南,新乡,453003 |
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| 基金项目： | 河南省基础与前沿研究项目(082300430030);创新人才工程项目(2005126-49);河南科技学院博士基金资助项目(HK05-1226) |
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| 摘 要： | Pink Peace’rose was used to study the optimum conditions for transferring the SeNHX1 gene into the callus. The results showed that the optimal medium was MS+2,4-D 5.0 mg·L-1 + TDZ 0.5 mg·L-1. Agrobacterium tumefaciens-mediated transformation was able to take the target gene into callus and the blue spots were found. The optimum conditions for the transient expression of gusA gene are as following: bacterium density of OD was 0.5, infection time was 20 min, culture time was 3 days. Adding 100 μmol·L-1 AS, the frequency of transient expression of GUS gene was the highest, which reached about 85% in present study.
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| 关 键 词： | 月季 遗传转化 农杆菌介导 SeNHX1基因 |
Studies on Agrobacterium-mediated Transformation of Rose Callus with SeNHX1 Gene |
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| LIU Hui-chao,LIU Meng-gang,GUO Li-juan and JIA Wen-qing.Studies on Agrobacterium-mediated Transformation of Rose Callus with SeNHX1 Gene[J].Forest Research,2010,23(4):617-621. |
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| Authors: | LIU Hui-chao LIU Meng-gang GUO Li-juan and JIA Wen-qing |
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| Abstract: | |
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| Keywords: | Rosa chinensis genetic transformation Agrobacterium-mediated SeNHX1 gene |
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