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| 不同地理株柔嫩艾美耳球虫(E.tenela) 杂交虫株(F1)的培育 | |
| 引用本文： | 赵权,张西臣,张伟信,尹继刚,李建华,杨贵连,徐朋,门静涛. 不同地理株柔嫩艾美耳球虫(E.tenela) 杂交虫株(F1)的培育[J]. 中国家禽, 2004, 8(Z1): 1-4 |
| 作者姓名： | 赵权 张西臣 张伟信 尹继刚 李建华 杨贵连 徐朋 门静涛 |
| 作者单位： | 1. 解放军军需大学军事兽医系,吉林,长春,130062;吉林农业大学动物科技学院,吉林,长春,130118 2. 解放军军需大学军事兽医系,吉林,长春,130062 3. 吉林农业大学动物科技学院,吉林,长春,130118 |
| 基金项目： | 国家自然科学基金项目(30170696) |
| 摘 要： | 通过用单卵囊接种技术培育了山东株(S)和黑龙江株(H),然后将山东株(S)和黑龙江株(H)的柔嫩艾美耳球虫的孢子化卵囊同时喂给雏鸡,收获卵囊,提取基因组DNA,再利用RAPD技术对它们基因组DNA进行分析,分析了19株柔嫩艾美耳球虫卵囊的DNA分子,确定了1株为山东株和黑龙江株的杂交株(F1)。这一研究结果为深入研究球虫的免疫机制奠定了基础。 |
| 关 键 词： | 柔嫩艾美耳球虫 杂交株 RAPD |
| 修稿时间： | 2004-11-22 |
Breed Hybridization F1 of Eimeria tenella from Different Area |
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| Zhao Quan,Zhang Xicheng,Zhang Weixin,Yin Jigang,Li Jianhua,Xu Pen,Men Jingtao,Yang Guilian. Breed Hybridization F1 of Eimeria tenella from Different Area[J]. China Poultry, 2004, 8(Z1): 1-4 | |
| Authors: | Zhao Quan Zhang Xicheng Zhang Weixin Yin Jigang Li Jianhua Xu Pen Men Jingtao Yang Guilian |
| Abstract: | 2 different strains of Emeria tenella were cultivated by single oocyst technology.They were Shandong strain (S)and Heilongjiang strain (H).The oocysts were fed chicken and receive oocysts.The gene DNA were extracted.Then the gene DNA were analysed by using RAPD.Oocyst DNA of 19 strains of E.tenella were tested,one strain were determined hybridization F1.This result offered base for immunity mechanism of E.tenella. |
| Keywords: | Eimeria tenella hybridization strain RAPD |
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