| 从陈旧血液提取基因组DNA的改进方法 |
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| 引用本文： | 古丽娜·艾山,木耶赛尔,马合木提·哈力克. 从陈旧血液提取基因组DNA的改进方法[J]. 畜禽业, 2010, 0(12) |
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| 作者姓名： | 古丽娜·艾山 木耶赛尔 马合木提·哈力克 |
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| 摘 要： | 目的:建立一种快速、有效、陈旧血样基因组DNA的提取方法。方法:采用SDS,EDTA,Tris,蛋白酶K作细胞裂解剂裂解细胞和细胞核,用Tris饱和酚和氯仿:异戊醇(24:1)直接提取基因组DNA。结果该方法提取陈旧血样基因组DNA的纯度高。结论:本法简便、有效,适用于批量陈旧血样的处理。
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| 关 键 词： | 基因组DNA 提取 陈旧血样 |
Blood extracted from the old improved method of genomic DNA |
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| Gulmira Hasan,Muyesar,Mahmut HALIK. Blood extracted from the old improved method of genomic DNA[J]. Livestock and Poultry Industry, 2010, 0(12) |
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| Authors: | Gulmira Hasan Muyesar Mahmut HALIK |
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| Abstract: | Objective:To esta blish a rapid,effective,old blood genomic DNA extraction method.Methods:SDS,EDTA,Tris,proteinase K cell lysis agent for cell lysis and nuclear,with Tris saturated phenol and chloroform:isoamyl alcohol(24:1) direct extraction of genomic DNA.Results The blood samples of genomic DNA from old high purity.Conclusion:This simple,effective and suitable for mass treatment of old blood samples. |
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| Keywords: | genomic DNA extraction old blood |
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